+検索条件
-Structure paper
| タイトル | The single-particle cryo-EM structures of a bacterial cyanide dihydratase and a fungal cyanide hydratase. |
|---|---|
| ジャーナル・号・ページ | Structure, Vol. 34, Issue 4, Page 599-610.e2, Year 2026 |
| 掲載日 | 2026年4月2日 |
著者 | Santiago Justo Arevalo / Valeria Valle-Riestra Felice / Mikaela Barahona Acuña / Katia Ordinola Flores / Mauro Quiñones Aguilar / Andrea Balan / Chuck Shaker Farah / ![]() |
| PubMed 要旨 | Cyanide is widely used in industries due to its affinity for metals, a property that also underlies its toxicity. Industries, therefore, must reduce cyanide concentration before the final disposal of ...Cyanide is widely used in industries due to its affinity for metals, a property that also underlies its toxicity. Industries, therefore, must reduce cyanide concentration before the final disposal of wastewater. Physical, chemical, and biological methods have been developed for this; however, knowledge about the structure of enzymes involved in cyanide degradation remains limited. Structural characterization of these proteins could facilitate the development of enzymes with enhanced bioremediation potential. Here, we present the single-particle cryo-electron microscopy structures of a cyanide dihydratase from Bacillus safensis and a cyanide hydratase from Gloeocercospora sorghi at 2.2 Å and 2.0 Å resolution, respectively. We provide a comprehensive description and comparative analysis alongside all previously determined nitrilase structures. Importantly, our full-length structures reveal new features in the C-terminal as well as specific intermolecular interactions between protomer interfaces and within the helix lumen. Finally, our findings offer insights into the reaction mechanisms of these two enzymes. |
リンク | Structure / PubMed:41709456 |
| 手法 | EM (単粒子) |
| 解像度 | 2.04 Å |
| 構造データ | EMDB-70376, PDB-9odt: EMDB-70421, PDB-9ofa: |
| 由来 |
|
キーワード | HYDROLASE / CynD / nitrilase / biorremediation / oligomer / CynH |
ムービー
コントローラー
構造ビューア
万見文献について



著者

リンク




microdochium sorghi (菌類)
キーワード