Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Immunofocusing on the conserved fusion peptide of HIV envelope glycoprotein in rhesus macaques.
Journal, issue, pages	bioRxiv, Year 2024
Publish date	Nov 29, 2024
Authors	Payal P Pratap / Christopher A Cottrell / James Quinn / Diane G Carnathan / Daniel L V Bader / Andy S Tran / Chiamaka A Enemuo / Julia T Ngo / Sara T Richey / Hongmei Gao / Xiaoying Shen / Kelli M Greene / Jonathan Hurtado / Katarzyna Kaczmarek Michaels / Elana Ben-Akiva / Joel D Allen / Gabriel Ozorowski / Max Crispin / Bryan Briney / David Montefiori / Guido Silvestri / Darrell J Irvine / Shane Crotty / Andrew B Ward
PubMed Abstract	During infection, the fusion peptide (FP) of HIV envelope glycoprotein (Env) serves a central role in viral fusion with the host cell. As such, the FP is highly conserved and therefore an attractive ...During infection, the fusion peptide (FP) of HIV envelope glycoprotein (Env) serves a central role in viral fusion with the host cell. As such, the FP is highly conserved and therefore an attractive epitope for vaccine design. Here, we describe a vaccination study in non-human primates (NHPs) where glycan deletions were made on soluble HIV Env to increase FP epitope exposure. When delivered via implantable osmotic pumps, this immunogen primed immune responses against the FP, which were then boosted with heterologous trimers resulting in a focused immune response targeting the conserved FP epitope. Although autologous immunizations did not elicit high affinity FP-targeting antibodies, the conserved FP epitope on a heterologous trimer further matured the lower affinity, FP-targeting B cells. This study suggests using epitope conservation strategies on distinct Env trimer immunogens can focus humoral responses on desired neutralizing epitopes and suppress immune-distracting antibody responses against non-neutralizing epitopes.
External links	bioRxiv / PubMed:39651156 / PubMed Central
Methods	EM (single particle)
Resolution	3.0 - 25.0 Å
Structure data	49411 9nhh EMDB-49411, PDB-9nhh: AMC016 v4.2 in complex with pAb Base-A isolated from animal RQk18 at week 43 Method: EM (single particle) / Resolution: 3.0 Å 49412 9nhi EMDB-49412, PDB-9nhi: AMC016 v4.2 in complex with Base-C pAb isolated from animal RQk18 at week 43 Method: EM (single particle) / Resolution: 3.1 Å 49413 9nhj EMDB-49413, PDB-9nhj: AMC016 v4.2 in complex with FP-A pAb from animal RQk18 at week 43 Method: EM (single particle) / Resolution: 3.04 Å 49414 9nhk EMDB-49414, PDB-9nhk: BG505-CH505 Env glycoprotein in complex with NHP pAb Base-4 isolated from animal RUu18 at week 14 Method: EM (single particle) / Resolution: 4.1 Å 49415 9nhl EMDB-49415, PDB-9nhl: BG505-CH505 Env glycoprotein in complex with NHP pAb FP-1 isolated from animal RUu18 at week 14 Method: EM (single particle) / Resolution: 3.7 Å 49416 9nhm EMDB-49416, PDB-9nhm: BG505-CH505 Env glycoprotein in complex with NHP pAb V1V2V3-1 isolated from animal RUu18 at week 14 Method: EM (single particle) / Resolution: 4.0 Å 49417 9nhn EMDB-49417, PDB-9nhn: BG505-CH505 Env glycoprotein in complex with NHP pAb V1V2V3-2 isolated from animal RUu18 at week 14 Method: EM (single particle) / Resolution: 3.9 Å 49418 9nho EMDB-49418, PDB-9nho: BG505-CH505 Env glycoprotein in complex with NHP pAb V1V2V3-5 isolated from animal RUu18 at week 14 Method: EM (single particle) / Resolution: 3.8 Å 49457 9ni9 EMDB-49457, PDB-9ni9: BG505-CH505 Env glycoprotein in complex with NHP pAb Base-1 isolated from animal RUu18 at week 14 Method: EM (single particle) / Resolution: 3.8 Å EMDB-70702: nsEM map of macaque RUu18 week 4 polyclonal Fab in complex with BG505-CH505+N241 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70703: nsEM map of macaque RMu18 week 4 polyclonal Fab in complex with BG505-CH505+N241 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70704: nsEM map of macaque RQk18 week 4 polyclonal Fab in complex with BG505-CH505dN241 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70705: nsEM map of macaque LJ66 week 4 polyclonal Fab in complex with BG505-CH505dN241 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70706: nsEM map of macaque RUu18 week 14 polyclonal Fab in complex with BG505-CH505+N241 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70707: nsEM map of macaque RMu18 week 14 polyclonal Fab in complex with BG505-CH505+N241 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70708: nsEM map of macaque RQk18 week 14 polyclonal Fab in complex with BG505-CH505+N241 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70709: nsEM map of macaque LJ66 week 14 polyclonal Fab in complex with BG505-CH505+N241 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70710: nsEM map of macaque RUu18 week 28 polyclonal Fab in complex with BG505-CH505+N241 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70711: nsEM map of macaque RMu18 week 28 polyclonal Fab in complex with BG505-CH505+N241 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70712: nsEM map of macaque RQk18 week 28 polyclonal Fab in complex with AMC016 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70713: nsEM map of macaque LJ66 week 28 polyclonal Fab in complex with AMC016 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70714: nsEM map of macaque RUu18 week 43 polyclonal Fab in complex with AMC016 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70715: nsEM map of macaque RMu18 week 43 polyclonal Fab in complex with AMC016 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70716: nsEM map of macaque RQk18 week 43 polyclonal Fab in complex with AMC016 Method: EM (single particle) / Resolution: 25.0 Å EMDB-70717: nsEM map of macaque LJ66 week 43 polyclonal Fab in complex with AMC016 Method: EM (single particle) / Resolution: 25.0 Å
Chemicals	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose
Source	human immunodeficiency virus 1 macaca mulatta (Rhesus monkey)
Keywords	VIRAL PROTEIN/IMMUNE SYSTEM / HIV-1 / polyclonal / cryoEMPEM / STRUCTURAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex