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基本情報
登録情報 | ![]() | |||||||||
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タイトル | BG505-CH505 Env glycoprotein in complex with NHP pAb Base-4 isolated from animal RUu18 at week 14 | |||||||||
![]() | EM Map Base4 from RUu18 week 14 | |||||||||
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![]() | HIV-1 / polyclonal / cryoEMPEM / STRUCTURAL PROTEIN | |||||||||
生物種 | ![]() ![]() ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.1 Å | |||||||||
![]() | Pratap PP / Ozorowski G / Ward AB | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Immunofocusing on the conserved fusion peptide of HIV envelope glycoprotein in rhesus macaques. 著者: Payal P Pratap / Christopher A Cottrell / James Quinn / Diane G Carnathan / Daniel L V Bader / Andy S Tran / Chiamaka A Enemuo / Julia T Ngo / Sara T Richey / Hongmei Gao / Xiaoying Shen / ...著者: Payal P Pratap / Christopher A Cottrell / James Quinn / Diane G Carnathan / Daniel L V Bader / Andy S Tran / Chiamaka A Enemuo / Julia T Ngo / Sara T Richey / Hongmei Gao / Xiaoying Shen / Kelli M Greene / Jonathan Hurtado / Katarzyna Kaczmarek Michaels / Elana Ben-Akiva / Joel D Allen / Gabriel Ozorowski / Max Crispin / Bryan Briney / David Montefiori / Guido Silvestri / Darrell J Irvine / Shane Crotty / Andrew B Ward 要旨: During infection, the fusion peptide (FP) of HIV envelope glycoprotein (Env) serves a central role in viral fusion with the host cell. As such, the FP is highly conserved and therefore an attractive ...During infection, the fusion peptide (FP) of HIV envelope glycoprotein (Env) serves a central role in viral fusion with the host cell. As such, the FP is highly conserved and therefore an attractive epitope for vaccine design. Here, we describe a vaccination study in non-human primates (NHPs) where glycan deletions were made on soluble HIV Env to increase FP epitope exposure. When delivered via implantable osmotic pumps, this immunogen primed immune responses against the FP, which were then boosted with heterologous trimers resulting in a focused immune response targeting the conserved FP epitope. Although autologous immunizations did not elicit high affinity FP-targeting antibodies, the conserved FP epitope on a heterologous trimer further matured the lower affinity, FP-targeting B cells. This study suggests using epitope conservation strategies on distinct Env trimer immunogens can focus humoral responses on desired neutralizing epitopes and suppress immune-distracting antibody responses against non-neutralizing epitopes. | |||||||||
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 259.4 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 23.8 KB 23.8 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 19.1 KB | 表示 | ![]() |
画像 | ![]() | 175.8 KB | ||
マスクデータ | ![]() | 274.6 MB | ![]() | |
Filedesc metadata | ![]() | 7.4 KB | ||
その他 | ![]() ![]() | 255.2 MB 255.2 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 937.5 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 937.1 KB | 表示 | |
XML形式データ | ![]() | 22.3 KB | 表示 | |
CIF形式データ | ![]() | 29.8 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
EMDBのページ | ![]() ![]() |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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注釈 | EM Map Base4 from RUu18 week 14 | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.15 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-マスク #1
ファイル | ![]() | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Half map A
ファイル | emd_49414_half_map_1.map | ||||||||||||
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注釈 | Half map A | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: half map b
ファイル | emd_49414_half_map_2.map | ||||||||||||
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注釈 | half map b | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
-全体 : BG505-CH505 Env glycoprotein in complex with NHP pAb Base-4 isola...
全体 | 名称: BG505-CH505 Env glycoprotein in complex with NHP pAb Base-4 isolated from animal RUu18 at week 14 |
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要素 |
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-超分子 #1: BG505-CH505 Env glycoprotein in complex with NHP pAb Base-4 isola...
超分子 | 名称: BG505-CH505 Env glycoprotein in complex with NHP pAb Base-4 isolated from animal RUu18 at week 14 タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#4 |
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-超分子 #2: BG505-CH505 Env glycoprotein
超分子 | 名称: BG505-CH505 Env glycoprotein / タイプ: complex / ID: 2 / 親要素: 1 / 含まれる分子: #3-#4 |
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由来(天然) | 生物種: ![]() ![]() |
-超分子 #3: pAb Base-4
超分子 | 名称: pAb Base-4 / タイプ: complex / ID: 3 / 親要素: 1 / 含まれる分子: #1-#2 |
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由来(天然) | 生物種: ![]() ![]() |
-分子 #1: RUu-Base-4 pAb heavy chain
分子 | 名称: RUu-Base-4 pAb heavy chain / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 10.298679 KDa |
配列 | 文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)C(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)W(UNK)(UNK) ...文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)C(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)W(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)C(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)W(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK) |
-分子 #2: RUu-Base-4 pAb light chain
分子 | 名称: RUu-Base-4 pAb light chain / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 9.202309 KDa |
配列 | 文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)W(UNK)(UNK)(UNK) ...文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)W(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)F(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) |
-分子 #3: BG505-CH505 Envelope glycoprotein gp120
分子 | 名称: BG505-CH505 Envelope glycoprotein gp120 / タイプ: protein_or_peptide / ID: 3 / コピー数: 3 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 56.228031 KDa |
組換発現 | 生物種: ![]() |
配列 | 文字列: MDAMKRGLCC VLLLCGAVFV SPSQEIHARF RRGARAENLW VTVYYGVPVW KDAETTLFCA SDAKAYETEK HNVWATHCCV PTDPNPQEI VLENVTENFN MWKNNMVEQM HEDIISLWDQ SLKPCVKLTP LCVTLNCTNA TASNSSIIEG MKNCSFNITT E LRDKREKK ...文字列: MDAMKRGLCC VLLLCGAVFV SPSQEIHARF RRGARAENLW VTVYYGVPVW KDAETTLFCA SDAKAYETEK HNVWATHCCV PTDPNPQEI VLENVTENFN MWKNNMVEQM HEDIISLWDQ SLKPCVKLTP LCVTLNCTNA TASNSSIIEG MKNCSFNITT E LRDKREKK NALFYKLDIV QLDGNSSQYR LINCNTSAIT QACPKVSFEP IPIHYCAPAG FAILKCNNKT FTGTGPCNNV ST VQCTHGI KPVVSTQLLL NGSLAEGEII IRSENITDNG KTILVHLNES VKIECTRPNN KTRTSIRIGP GQAFYATGQV IGD IREAYC NISESTWNET LGKVVKQLRK HFPHKNITFQ PSSGGDLEVT THSFNCGGEF FYCNTSGLFN STWISNTSVQ GSNS TGSND SITLPCRIKQ IINMWQEVGR AMYAPPIQGN ITCVSNITGL ILTRDGGKNN TETFRPGGGD MRDNWRSELY KYKVV KIEP LGVAPTACKR RVVGRRRRRR |
-分子 #4: BG505-CH505 Transmembrane protein gp41
分子 | 名称: BG505-CH505 Transmembrane protein gp41 / タイプ: protein_or_peptide / ID: 4 / コピー数: 3 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 17.16535 KDa |
組換発現 | 生物種: ![]() |
配列 | 文字列: AVGIGAVFLG FLGAAGSTMG AASMTLTVQA RNLLSGIVQQ QSNLLRAPEC QQHLLKDTHW GIKQLQARVL AVEHYLRDQQ LLGIWGCSG KLICTTNVPW NSTWSNKTLS EIWDNMTWLQ WDKEISNYTQ IIYGLLEESQ NQQEKNETDN LTCD |
-分子 #8: 2-acetamido-2-deoxy-beta-D-glucopyranose
分子 | 名称: 2-acetamido-2-deoxy-beta-D-glucopyranose / タイプ: ligand / ID: 8 / コピー数: 55 / 式: NAG |
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分子量 | 理論値: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 構成要素:
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グリッド | モデル: Quantifoil R1.2/1.3 / 材質: COPPER / メッシュ: 400 / 前処理 - タイプ: PLASMA CLEANING | ||||||||
凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TALOS ARCTICA |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 実像数: 7167 / 平均電子線量: 49.92 e/Å2 |
電子線 | 加速電圧: 200 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.0 µm / 最小 デフォーカス(公称値): 1.0 µm / 倍率(公称値): 36000 |
試料ステージ | ホルダー冷却材: NITROGEN |
実験機器 | ![]() モデル: Talos Arctica / 画像提供: FEI Company |