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Structure paper

TitleMolecular mechanisms of urate transport by the native human URAT1 and its inhibition by anti-gout drugs.
Journal, issue, pagesCell Discov, Vol. 11, Issue 1, Page 33, Year 2025
Publish dateApr 1, 2025
AuthorsCanrong Wu / Chao Zhang / Sanshan Jin / James Jiqi Wang / Antao Dai / Jiuyin Xu / Heng Zhang / Xuemei Yang / Xinheng He / Qingning Yuan / Wen Hu / Youwei Xu / Mingwei Wang / Yi Jiang / Dehua Yang / H Eric Xu /
PubMed AbstractGout, a common and painful disease, stems from hyperuricemia, where elevated blood urate levels lead to urate crystal formation in joints and kidneys. The human urate transporter 1 (hURAT1) plays a ...Gout, a common and painful disease, stems from hyperuricemia, where elevated blood urate levels lead to urate crystal formation in joints and kidneys. The human urate transporter 1 (hURAT1) plays a critical role in urate homeostasis by facilitating urate reabsorption in the renal proximal tubule, making it a key target for gout therapy. Pharmacological inhibition of hURAT1 with drugs such as dotinurad, benzbromarone, lesinurad, and verinurad promotes urate excretion and alleviates gout symptoms. Here, we present cryo-electron microscopy structures of native hURAT1 bound with these anti-gout drugs in the inward-open state, and with urate in inward-open, outward-open, and occluded states. Complemented by mutagenesis and cell-based assays, these structures reveal the mechanisms of urate reabsorption and hURAT1 inhibition. Our findings elucidate the molecular basis of urate transport and anti-gout medication action and provide a structural framework for the rational design of next-generation therapies for hyperuricemia and gout.
External linksCell Discov / PubMed:40169562 / PubMed Central
MethodsEM (single particle)
Resolution3.23 - 3.6 Å
Structure data

61399

9jdv

EMDB-61399, PDB-9jdv:
Human URAT1 bound with Uric acid
Method: EM (single particle) / Resolution: 3.32 Å

61401

9jdy

EMDB-61401, PDB-9jdy:
Human URAT1 bound with verinurad
Method: EM (single particle) / Resolution: 3.23 Å

61402

9jdz

EMDB-61402, PDB-9jdz:
Human URAT1 bound to lesinurad
Method: EM (single particle) / Resolution: 3.5 Å

61403

9je0

EMDB-61403, PDB-9je0:
Human URAT1 bound to benzbromarone
Method: EM (single particle) / Resolution: 3.23 Å

61404

9je1

EMDB-61404, PDB-9je1:
Human URAT1 bound to dotinurad
Method: EM (single particle) / Resolution: 3.6 Å

Chemicals

ChemComp-URC:
URIC ACID

ChemComp-HOH:
WATER

PDB-1aij:
PHOTOSYNTHETIC REACTION CENTER FROM RHODOBACTER SPHAEROIDES IN THE CHARGE-NEUTRAL DQAQB STATE

PDB-1ail:
N-TERMINAL FRAGMENT OF NS1 PROTEIN FROM INFLUENZA A VIRUS

ChemComp-R75:
[3,5-bis(bromanyl)-4-oxidanyl-phenyl]-(2-ethyl-1-benzofuran-3-yl)methanone / antiarrhythmic, inhibitor*YM

PDB-1aik:
HIV GP41 CORE STRUCTURE

Source
  • homo sapiens (human)
KeywordsTRANSPORT PROTEIN / URAT1

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