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TitleCryo-EM structure of the bacterial intramembrane metalloprotease RseP in the substrate-bound state.
Journal, issue, pagesSci Adv, Vol. 11, Issue 9, Page eadu0925, Year 2025
Publish dateFeb 28, 2025
AuthorsKikuko Asahi / Mika Hirose / Rie Aruga / Yosuke Shimizu / Michiko Tajiri / Tsubasa Tanaka / Yuriko Adachi / Yukari Tanaka / Mika K Kaneko / Yukinari Kato / Satoko Akashi / Yoshinori Akiyama / Yohei Hizukuri / Takayuki Kato / Terukazu Nogi /
PubMed AbstractSite-2 proteases (S2Ps), conserved intramembrane metalloproteases that maintain cellular homeostasis, are associated with chronic infection and persistence leading to multidrug resistance in ...Site-2 proteases (S2Ps), conserved intramembrane metalloproteases that maintain cellular homeostasis, are associated with chronic infection and persistence leading to multidrug resistance in bacterial pathogens. A structural model of how S2Ps discriminate and accommodate substrates could help us develop selective antimicrobial agents. We previously proposed that the S2P RseP unwinds helical substrate segments before cleavage, but the mechanism for accommodating a full-length membrane-spanning substrate remained unclear. Our present cryo-EM analysis of RseP (RseP) revealed that a substrate-like membrane protein fragment from the expression host occupied the active site while spanning a transmembrane cavity that is inaccessible via lateral diffusion. Furthermore, in vivo photocrosslinking supported that this substrate accommodation mode is recapitulated on the cell membrane. Our results suggest that the substrate accommodation by threading through a conserved membrane-associated region stabilizes the substrate-complex and contributes to substrate discrimination on the membrane.
External linksSci Adv / PubMed:40009668 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.3 - 3.95 Å
Structure data

61213

9j82

EMDB-61213, PDB-9j82:
Cryo-EM structure of wild type Aquifex aeolicus RseP in complex with Fab
Method: EM (single particle) / Resolution: 3.95 Å

61214

9j83

EMDB-61214, PDB-9j83:
Cryo-EM structure of Aquifex aeolicus RseP E18Q mutant in complex with Fab
Method: EM (single particle) / Resolution: 3.61 Å

PDB-8zay:
Crystal structure of Fab from mouse monoclonal antibody 4A9 against Aquifex aeolicus RseP PDZ tandem
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • mus musculus (house mouse)
  • Aquifex aeolicus (strain VF5) (bacteria)
  • aquifex aeolicus vf5 (bacteria)
  • escherichia coli (E. coli)
KeywordsPROTEIN BINDING / Antibody / HYDROLASE / Protease / Intramembrane protease

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