EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Unidirectional MCM translocation away from ORC drives origin licensing.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 782, Year 2025
掲載日	2025年1月17日
著者	Agata Butryn / Julia F Greiwe / Alessandro Costa /
PubMed 要旨	The MCM motor of the eukaryotic replicative helicase is loaded as a double hexamer onto DNA by the Origin Recognition Complex (ORC), Cdc6, and Cdt1. ATP binding supports formation of the ORC-Cdc6- ...The MCM motor of the eukaryotic replicative helicase is loaded as a double hexamer onto DNA by the Origin Recognition Complex (ORC), Cdc6, and Cdt1. ATP binding supports formation of the ORC-Cdc6-Cdt1-MCM (OCCM) helicase-recruitment complex where ORC-Cdc6 and one MCM hexamer form two juxtaposed rings around duplex DNA. ATP hydrolysis by MCM completes MCM loading but the mechanism is unknown. Here, we used cryo-EM to characterise helicase loading with ATPase-dead Arginine Finger variants of the six MCM subunits. We report the structure of two MCM complexes with different DNA grips, stalled as they mature to loaded MCM. The Mcm2 Arginine Finger-variant stabilises DNA binding by Mcm2 away from ORC/Cdc6. The Arginine Finger-variant of the neighbouring Mcm5 subunit stabilises DNA engagement by Mcm5 downstream of the Mcm2 binding site. Cdc6 and Orc1 progressively disengage from ORC as MCM translocates along DNA. We observe that duplex DNA translocation by MCM involves a set of leading-strand contacts by the pre-sensor 1 ATPase hairpins and lagging-strand contacts by the helix-2-insert hairpins. Mutating any of the MCM residues involved impairs high-salt resistant DNA binding in vitro and double-hexamer formation assessed by electron microscopy. Thus, ATPase-powered duplex DNA translocation away from ORC underlies MCM loading.
リンク	Nat Commun / PubMed:39824870 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 3.7 Å
構造データ	EMDB-51398: OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm5: Conformer 2. Consensus map. 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-51399: OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm5: Conformer 2. ORC local refinement. 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-51400: OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm5: Conformer 2. Local MCM-Cdt1 refinement. 手法: EM (単粒子) / 解像度: 3.5 Å 51401 9gjp EMDB-51401: OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm5: Conformer 2. Composite map. PDB-9gjp: OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm5: Conformer 2 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-51404: OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm2. Consensus map 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-51405: OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm2. ORC local refinement. 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-51406: OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm2. MCM-Cdt1 local refinement. 手法: EM (単粒子) / 解像度: 3.2 Å 51407 9gjw EMDB-51407: OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm2. Composite map. PDB-9gjw: OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm2 手法: EM (単粒子) / 解像度: 3.3 Å 51441 9gm5 EMDB-51441, PDB-9gm5: OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm5: Conformer 1 手法: EM (単粒子) / 解像度: 3.7 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-ZN: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM
由来	saccharomyces cerevisiae (パン酵母) synthetic construct (人工物)
キーワード	REPLICATION / AAA+ ATPase / DNA translocase / ATPase switch / DNA replication