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TitleNew rhinovirus uncoating intermediate reveals how sodium versus potassium ions influence RNA release.
Journal, issue, pagesSci Rep, Vol. 15, Issue 1, Page 36768, Year 2025
Publish dateOct 21, 2025
AuthorsAntonio Real-Hohn / Dieter Blaas /
PubMed AbstractElectron microscopy (EM) of rhinovirus A2 (RV-A2) incubated in Na phosphate buffer (pH 7.6) for 12 h at 25 °C revealed partial fragmentation, whereas upon incubation in K phosphate buffer, RV-A2 ...Electron microscopy (EM) of rhinovirus A2 (RV-A2) incubated in Na phosphate buffer (pH 7.6) for 12 h at 25 °C revealed partial fragmentation, whereas upon incubation in K phosphate buffer, RV-A2 appeared intact. In buffers adjusted to pH 5.8, these differences became more pronounced; acidic Na phosphate buffer promoted disintegration of the particles, whereas in acidic K phosphate buffer, the virus appeared like native. Incubation in the acidic buffers for one hour at 4 °C followed by neutralisation resulted in the respective formation of non-infectious A particles (in Na) and a non-infectious novel uncoating intermediate (in K), which we termed 'E0 particle'. Negative staining EM revealed phosphotungstate penetration into A particles, but not into E0 particles. Cryo-EM image reconstruction of the E0 particle showed clear differences between A and E0 particles; like native virus, E0 contained VP4 and a pocket factor. Native RV-A2 RNA cores, obtained by gentle proteinase-K digestion in K and Na phosphate buffer, respectively, differed in accessibility of dsRNA regions, detected by PaSTRy. Variance in RNA compactness observed in K versus Na phosphate buffer was confirmed by rotary shadowing EM; in K phosphate buffer, the RNA remained condensed while, in Na phosphate buffer, distinct unfolding stages were apparent.
External linksSci Rep / PubMed:41120457 / PubMed Central
MethodsEM (single particle)
Resolution3.2 Å
Structure data

50930

9g0b

EMDB-50930, PDB-9g0b:
Rhinovirus A2 uncoating intermediate revealing the natural pocket factor (pH 5.8 and 4 degrees Celsius)
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-DAO:
LAURIC ACID

Source
  • rhinovirus a2
KeywordsVIRUS / Uncoating Intermediate / Pocket Factor / Lauric Acid

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