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TitleNovel archaeal ribosome dimerization factor facilitating unique 30S-30S dimerization.
Journal, issue, pagesNucleic Acids Res, Vol. 53, Issue 2, Year 2025
Publish dateJan 11, 2025
AuthorsAhmed H Hassan / Matyas Pinkas / Chiaki Yaeshima / Sonoko Ishino / Toshio Uchiumi / Kosuke Ito / Gabriel Demo /
PubMed AbstractProtein synthesis (translation) consumes a substantial proportion of cellular resources, prompting specialized mechanisms to reduce translation under adverse conditions. Ribosome inactivation often ...Protein synthesis (translation) consumes a substantial proportion of cellular resources, prompting specialized mechanisms to reduce translation under adverse conditions. Ribosome inactivation often involves ribosome-interacting proteins. In both bacteria and eukaryotes, various ribosome-interacting proteins facilitate ribosome dimerization or hibernation, and/or prevent ribosomal subunits from associating, enabling the organisms to adapt to stress. Despite extensive studies on bacteria and eukaryotes, understanding factor-mediated ribosome dimerization or anti-association in archaea remains elusive. Here, we present cryo-electron microscopy structures of an archaeal 30S dimer complexed with an archaeal ribosome dimerization factor (designated aRDF), from Pyrococcus furiosus, resolved at a resolution of 3.2 Å. The complex features two 30S subunits stabilized by aRDF homodimers in a unique head-to-body architecture, which differs from the disome architecture observed during hibernation in bacteria and eukaryotes. aRDF interacts directly with eS32 ribosomal protein, which is essential for subunit association. The binding mode of aRDF elucidates its anti-association properties, which prevent the assembly of archaeal 70S ribosomes.
External linksNucleic Acids Res / PubMed:39797736 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 3.4 Å
Structure data

50611

9fny

EMDB-50611, PDB-9fny:
PF30S-PF30S dimer mediated by aRDF from P. furiosus (Structure I)
Method: EM (single particle) / Resolution: 3.2 Å

50612

9fnz

EMDB-50612, PDB-9fnz:
PF30S-PF30S dimer mediated by aRDF from P. furiosus (Structure II)
Method: EM (single particle) / Resolution: 3.2 Å

50613

9fo0

EMDB-50613, PDB-9fo0:
PF30S ribosomal subunit - control
Method: EM (single particle) / Resolution: 3.4 Å

Source
  • pyrococcus furiosus (archaea)
KeywordsTRANSLATION / Pyrococcus furiosus / ribosomal subunit / dimerization / anti-association / ribosome-associated protein

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