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TitleA nanoengineered tandem nitroreductase: designing a robust prodrug-activating nanoreactor.
Journal, issue, pagesRSC Chem Biol, Vol. 6, Issue 1, Page 21-35, Year 2024
Publish dateNov 4, 2024
AuthorsMariia Zmyslia / Michael J Capper / Michael Grimmeisen / Kerstin Sartory / Benedikt Deuringer / Mohamed Abdelsalam / Kaiwei Shen / Manfred Jung / Wolfgang Sippl / Hans-Georg Koch / Laurine Kaul / Regine Süss / Jesko Köhnke / Claudia Jessen-Trefzer /
PubMed AbstractNitroreductases are important enzymes for a variety of applications, including cancer therapy and bioremediation. They often require encapsulation to improve stability and activity. We focus on ...Nitroreductases are important enzymes for a variety of applications, including cancer therapy and bioremediation. They often require encapsulation to improve stability and activity. We focus on genetically encoded encapsulation of nitroreductases within protein capsids, like encapsulins. Our study showcases the encapsulation of nitroreductase NfsB as functional dimers within encapsulins, which enhances protein activity and stability in diverse conditions. Mutations within the pore region are beneficial for activity of the encapsulated enzyme, potentially by increasing diffusion rates. Cryogenic electron microscopy reveals the overall architecture of the encapsulated dimeric NfsB within the nanoreactor environment and identifies multiple pore states in the shell. These findings highlight the potential of encapsulins as versatile tools for enhancing enzyme performance across various fields.
External linksRSC Chem Biol / PubMed:39508026 / PubMed Central
MethodsEM (single particle)
Resolution2.22 - 2.81 Å
Structure data

50585

9fn9

EMDB-50585, PDB-9fn9:
Icosahedral Encapsulin with a closed pore state
Method: EM (single particle) / Resolution: 2.81 Å

50586

9fna

EMDB-50586, PDB-9fna:
CryoEM structure of Encapsulin::tdNfsB with an open pore state
Method: EM (single particle) / Resolution: 2.22 Å

Source
  • mycolicibacterium smegmatis (bacteria)
  • mycolicibacterium smegmatis mc2 155 (bacteria)
KeywordsBIOSYNTHETIC PROTEIN / Nanocompartment

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