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Structure paper

TitleStructural basis of voltage-dependent gating in BK channels.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 5846, Year 2025
Publish dateJul 1, 2025
AuthorsGustavo F Contreras / Rong Shen / Ramon Latorre / Eduardo Perozo /
PubMed AbstractThe allosteric communication between the pore domain, voltage sensors, and Ca binding sites in the calcium- and voltage-activated K channel (BK) underlies its physiological role as the preeminent ...The allosteric communication between the pore domain, voltage sensors, and Ca binding sites in the calcium- and voltage-activated K channel (BK) underlies its physiological role as the preeminent signal integrator in excitable systems. BK displays shallow voltage sensitivity with very fast gating charge kinetics, yet little is known about the molecular underpinnings of this distinctive behavior. Here, we explore the mechanistic basis of coupling between voltage-sensing domains (VSDs) and calcium sensors in Aplysia BK by locking the VSDs in their activated (R196Q and R199Q) and resting (R202Q) states, with or without calcium. Cryo-EM structures of these mutants reveal unique tilts at the S4 C-terminal end, together with large side-chain rotameric excursions of the gating charges. Notably, the VSD resting structure (R202Q) also revealed BK in its elusive, fully closed state, highlighting the reciprocal relation between calcium and voltage sensors. These structures provide a plausible path where voltage and Ca binding couple energetically and define the conformation of the pore domain and, thus, BK's full functional range.
External linksNat Commun / PubMed:40593533 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 3.5 Å
Structure data

46901

9di8

EMDB-46901, PDB-9di8:
Ca2+ bound aplysia Slo1 - R196Q
Method: EM (single particle) / Resolution: 3.1 Å

46903

9dic

EMDB-46903, PDB-9dic:
Apo aplysia Slo1 - R196Q
Method: EM (single particle) / Resolution: 2.8 Å

46918

9dit

EMDB-46918, PDB-9dit:
Ca2+ bound aplysia Slo1 R199Q
Method: EM (single particle) / Resolution: 2.9 Å

46939

9djv

EMDB-46939, PDB-9djv:
apo aplysia Slo1 R199Q
Method: EM (single particle) / Resolution: 3.4 Å

46956

9dkf

EMDB-46956, PDB-9dkf:
Ca2+ bound aplysia Slo1 R202Q
Method: EM (single particle) / Resolution: 2.6 Å

46961

9dkl

EMDB-46961, PDB-9dkl:
Ca2+ bound aplysia Slo1 F304A
Method: EM (single particle) / Resolution: 3.4 Å

46963

9dkn

EMDB-46963, PDB-9dkn:
Apo aplysia Slo1 R202Q
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-K:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-CA:
Unknown entry

Source
  • aplysia californica (California sea hare)
KeywordsMEMBRANE PROTEIN / Potassium Channel / Voltage sensor / calcium bound / open channel / calcium-activated / large conductance / transport protein

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