EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for intrinsic strand displacement activity of mitochondrial DNA polymerase.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 2417, Year 2025
掲載日	2025年3月11日
著者	Ashok R Nayak / Viktoriia Sokolova / Sirelin Sillamaa / Karl Herbine / Juhan Sedman / Dmitry Temiakov /
PubMed 要旨	Members of the Pol A family of DNA polymerases, found across all domains of life, utilize various strategies for DNA strand separation during replication. In higher eukaryotes, mitochondrial DNA ...Members of the Pol A family of DNA polymerases, found across all domains of life, utilize various strategies for DNA strand separation during replication. In higher eukaryotes, mitochondrial DNA polymerase γ relies on the replicative helicase TWINKLE, whereas the yeast ortholog, Mip1, can unwind DNA independently. Using Mip1 as a model, we present a series of high-resolution cryo-EM structures that capture the process of DNA strand displacement. Our data reveal previously unidentified structural elements that facilitate the unwinding of the downstream DNA duplex. Yeast cells harboring Mip1 variants defective in strand displacement exhibit impaired oxidative phosphorylation and loss of mtDNA, corroborating the structural observations. This study provides a molecular basis for the intrinsic strand displacement activity of Mip1 and illuminates the distinct unwinding mechanisms utilized by Pol A family DNA polymerases.
リンク	Nat Commun / PubMed:40069189 / PubMed Central
手法	EM (単粒子)
解像度	2.42 - 2.96 Å
構造データ	45194 9c51 EMDB-45194, PDB-9c51: Cryo-EM structure of the Strand displacement Complex (IV) of Yeast Mitochondrial DNA polymerase Gamma (MIP1) with downstream DNA 手法: EM (単粒子) / 解像度: 2.42 Å 45195 9c52 EMDB-45195, PDB-9c52: Cryo-EM structure of the Strand displacement Complex (I) of Yeast Mitochondrial DNA polymerase Gamma (MIP1) with downstream DNA 手法: EM (単粒子) / 解像度: 2.64 Å 45196 9c53 EMDB-45196: Cryo-EM structure of the Strand displacement Complex (III) of Yeast Mitochondrial DNA polymerase Gamma (MIP1) with downstream DNA PDB-9c53: Cryo-EM structure of the Strand displacement Complex (II) of Yeast Mitochondrial DNA polymerase Gamma (MIP1) with downstream DNA 手法: EM (単粒子) / 解像度: 2.6 Å 46497 9d2i EMDB-46497, PDB-9d2i: Cryo-EM structure of the Strand displacement Complex (III) of Yeast Mitochondrial DNA polymerase Gamma (MIP1) with downstream DNA 手法: EM (単粒子) / 解像度: 2.96 Å
化合物	ChemComp-DTP: 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE ChemComp-MG: Unknown entry ChemComp-2DT: 3'-DEOXYTHYMIDINE-5'-MONOPHOSPHATE
由来	saccharomyces cerevisiae (パン酵母) synthetic construct (人工物)
キーワード	Transferase/DNA / Mitochondrial DNA Polymerase Gamma / Strand displacement complex / MIP1 / REPLICATION / Helicase independent DNA polymerase / Transferase-DNA complex