Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Mechanisms underlying modulation of human GlyRα3 by Zn and pH.
Journal, issue, pages	Sci Adv, Vol. 10, Issue 51, Page eadr5920, Year 2024
Publish date	Dec 20, 2024
Authors	Kayla Kindig / Eric Gibbs / David Seiferth / Philip C Biggin / Sudha Chakrapani /
PubMed Abstract	Glycine receptors (GlyRs) regulate motor control and pain processing in the central nervous system through inhibitory synaptic signaling. The subtype GlyRα3 expressed in nociceptive sensory neurons ...Glycine receptors (GlyRs) regulate motor control and pain processing in the central nervous system through inhibitory synaptic signaling. The subtype GlyRα3 expressed in nociceptive sensory neurons of the spinal dorsal horn is a key regulator of physiological pain perception. Disruption of spinal glycinergic inhibition is associated with chronic inflammatory pain states, making GlyRα3 an attractive target for pain treatment. GlyRα3 activity is modulated by numerous endogenous and exogenous ligands that consequently affect pain sensitization. To understand the mechanism of two such endogenous modulators, Zn and protons, we have used cryo-electron microscopy to determine structures of full-length human GlyRα3 in various functional states. Whereas acidic pH reduces peak glycine response, Zn displays biphasic modulation in a concentration-dependent manner. Our findings reveal the effector sites and also capture intermediate conformations in the gating cycle. Combined with molecular dynamics simulations and electrophysiology, this work provides important insights into GlyRα3 activation and regulation.
External links	Sci Adv / PubMed:39693447 / PubMed Central
Methods	EM (single particle)
Resolution	2.19 - 3.07 Å
Structure data	44899 9bu2 EMDB-44899, PDB-9bu2: Homomeric alpha3 glycine receptor in the presence of 1 mM glycine at pH 6.4 in a desensitized state Method: EM (single particle) / Resolution: 2.87 Å 44900 9bu3 EMDB-44900, PDB-9bu3: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine in a desensitized state Method: EM (single particle) / Resolution: 2.8 Å 44933 9bvh EMDB-44933, PDB-9bvh: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine in an apo state. Method: EM (single particle) / Resolution: 2.58 Å 44934 9bvj EMDB-44934, PDB-9bvj: Homomeric alpha3 glycine receptor in the presence of 1 mM glycine in an desensitized state. Method: EM (single particle) / Resolution: 2.8 Å 44963 9bwb EMDB-44963, PDB-9bwb: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine at pH 6.4 in a desensitized state Method: EM (single particle) / Resolution: 2.21 Å 44964 9bwc EMDB-44964, PDB-9bwc: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine at pH 6.4 in an apo state Method: EM (single particle) / Resolution: 2.19 Å 44970 9bwe EMDB-44970, PDB-9bwe: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine at pH 6.4 in an intermediate state Method: EM (single particle) / Resolution: 3.07 Å 44974 9bwg EMDB-44974, PDB-9bwg: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine and 0.1 mM zinc in a desensitized state Method: EM (single particle) / Resolution: 2.59 Å 44976 9bwj EMDB-44976, PDB-9bwj: Homomeric alpha3 glycine receptor in the presence of 0.1 mM glycine and 0.1 mM zinc in an apo state Method: EM (single particle) / Resolution: 2.21 Å 45073 9bzp EMDB-45073, PDB-9bzp: Homomeric alpha3 glycine receptor in the presence of 0.1 millimolar glycine and 1 micromolar zinc in a desensitized state Method: EM (single particle) / Resolution: 2.88 Å
Chemicals	ChemComp-GLY: GLYCINE ChemComp-PIO: [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate ChemComp-PX4: 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / DMPC, phospholipidYM ChemComp-HOH: WATER ChemComp-CLR: CHOLESTEROL ChemComp-ZN*: Unknown entry
Source	homo sapiens (human)
Keywords	MEMBRANE PROTEIN / Glycine / Ion Channel / Ligand-Gated / Pentameric