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TitleP4-ATPases control phosphoinositide membrane asymmetry and neomycin resistance.
Journal, issue, pagesNat Cell Biol, Vol. 27, Issue 7, Page 1114-1124, Year 2025
Publish dateJul 11, 2025
AuthorsBhawik K Jain / H Diessel Duan / Christina Valentine / Ariana Samiha / Huilin Li / Todd R Graham /
PubMed AbstractThe aminoglycoside antibiotic neomycin has robust antibacterial properties, yet its clinical utility is curtailed by its nephrotoxicity and ototoxicity. The mechanism by which the polycationic ...The aminoglycoside antibiotic neomycin has robust antibacterial properties, yet its clinical utility is curtailed by its nephrotoxicity and ototoxicity. The mechanism by which the polycationic neomycin enters specific eukaryotic cell types remains poorly understood. In budding yeast, NEO1 is required for neomycin resistance and encodes a phospholipid flippase that establishes membrane asymmetry. Here we show that mutations altering Neo1 substrate recognition cause neomycin hypersensitivity by exposing phosphatidylinositol-4-phosphate (PI4P) in the plasma membrane extracellular leaflet. Cryogenic electron microscopy reveals PI4P binding to Neo1 within the substrate translocation pathway. PI4P enters the lumen of the endoplasmic reticulum and is flipped by Neo1 at the Golgi to prevent PI4P secretion to the cell surface. Deficiency of the orthologous ATP9A in human cells also causes exposure of PI4P and neomycin sensitivity. These findings unveil conserved mechanisms of aminoglycoside sensitivity and phosphoinositide homoeostasis, with important implications for signalling by extracellular phosphoinositides.
External linksNat Cell Biol / PubMed:40646185 / PubMed Central
MethodsEM (single particle)
Resolution3.71 Å
Structure data

44850

9bs1

EMDB-44850, PDB-9bs1:
Cryo-EM structure of the S. cerevisiae lipid flippase Neo1 bound with PI4P in the E2P state
Method: EM (single particle) / Resolution: 3.71 Å

Chemicals

ChemComp-BEF:
BERYLLIUM TRIFLUORIDE ION

PDB-1arj:
ARG-BOUND TAR RNA, NMR

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsLIPID TRANSPORT / lipid flippase / P4-ATPase / PI4P / phosphoinositide / neomycin resistance

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