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TitlePore Engineering as a General Strategy to Improve Protein-Based Enzyme Nanoreactor Performance.
Journal, issue, pagesACS Nano, Vol. 18, Issue 37, Page 25740-25753, Year 2024
Publish dateSep 17, 2024
AuthorsSeokmu Kwon / Michael P Andreas / Tobias W Giessen /
PubMed AbstractEnzyme nanoreactors are nanoscale compartments consisting of encapsulated enzymes and a selectively permeable barrier. Sequestration and colocalization of enzymes can increase catalytic activity, ...Enzyme nanoreactors are nanoscale compartments consisting of encapsulated enzymes and a selectively permeable barrier. Sequestration and colocalization of enzymes can increase catalytic activity, stability, and longevity, highly desirable features for many biotechnological and biomedical applications of enzyme catalysts. One promising strategy to construct enzyme nanoreactors is to repurpose protein nanocages found in nature. However, protein-based enzyme nanoreactors often exhibit decreased catalytic activity, partially caused by a mismatch of protein shell selectivity and the substrate requirements of encapsulated enzymes. No broadly applicable and modular protein-based nanoreactor platform is currently available. Here, we introduce a pore-engineered universal enzyme nanoreactor platform based on encapsulins-microbial self-assembling protein nanocompartments with programmable and selective enzyme packaging capabilities. We structurally characterize our protein shell designs via cryo-electron microscopy and highlight their polymorphic nature. Through fluorescence polarization assays, we show their improved molecular flux behavior and highlight their expanded substrate range via a number of proof-of-concept enzyme nanoreactor designs. This work lays the foundation for utilizing our encapsulin-based nanoreactor platform for diverse future biotechnological and biomedical applications.
External linksACS Nano / PubMed:39226211
MethodsEM (single particle)
Resolution2.86 - 3.14 Å
Structure data

44383

9b9i

EMDB-44383, PDB-9b9i:
Myxococcus xanthus EncA encapsulin engineered pore mutant with T=1 icosahedral symmetry
Method: EM (single particle) / Resolution: 2.86 Å

44388

9b9q

EMDB-44388, PDB-9b9q:
Cargo-loaded Myxococcus xanthus EncA encapsulin engineered pore mutant with T=3 icosahedral symmetry
Method: EM (single particle) / Resolution: 3.14 Å

Source
  • myxococcus xanthus dk 1622 (bacteria)
KeywordsVIRUS LIKE PARTICLE / encapsulin / nanocompartment / pore mutant

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