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タイトル | Pore Engineering as a General Strategy to Improve Protein-Based Enzyme Nanoreactor Performance. |
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ジャーナル・号・ページ | ACS Nano, Vol. 18, Issue 37, Page 25740-25753, Year 2024 |
掲載日 | 2024年9月17日 |
著者 | Seokmu Kwon / Michael P Andreas / Tobias W Giessen / |
PubMed 要旨 | Enzyme nanoreactors are nanoscale compartments consisting of encapsulated enzymes and a selectively permeable barrier. Sequestration and colocalization of enzymes can increase catalytic activity, ...Enzyme nanoreactors are nanoscale compartments consisting of encapsulated enzymes and a selectively permeable barrier. Sequestration and colocalization of enzymes can increase catalytic activity, stability, and longevity, highly desirable features for many biotechnological and biomedical applications of enzyme catalysts. One promising strategy to construct enzyme nanoreactors is to repurpose protein nanocages found in nature. However, protein-based enzyme nanoreactors often exhibit decreased catalytic activity, partially caused by a mismatch of protein shell selectivity and the substrate requirements of encapsulated enzymes. No broadly applicable and modular protein-based nanoreactor platform is currently available. Here, we introduce a pore-engineered universal enzyme nanoreactor platform based on encapsulins-microbial self-assembling protein nanocompartments with programmable and selective enzyme packaging capabilities. We structurally characterize our protein shell designs via cryo-electron microscopy and highlight their polymorphic nature. Through fluorescence polarization assays, we show their improved molecular flux behavior and highlight their expanded substrate range via a number of proof-of-concept enzyme nanoreactor designs. This work lays the foundation for utilizing our encapsulin-based nanoreactor platform for diverse future biotechnological and biomedical applications. |
リンク | ACS Nano / PubMed:39226211 |
手法 | EM (単粒子) |
解像度 | 2.86 - 3.14 Å |
構造データ | EMDB-44383, PDB-9b9i: EMDB-44388, PDB-9b9q: |
由来 |
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キーワード | VIRUS LIKE PARTICLE / encapsulin / nanocompartment / pore mutant |