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-Structure paper
| タイトル | Structural insight into sodium ion pathway in the bacterial flagellar stator from marine . |
|---|---|
| ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 122, Issue 1, Page e2415713122, Year 2025 |
| 掲載日 | 2025年1月7日 |
著者 | Tatsuro Nishikino / Norihiro Takekawa / Jun-Ichi Kishikawa / Mika Hirose / Seiji Kojima / Michio Homma / Takayuki Kato / Katsumi Imada / ![]() |
| PubMed 要旨 | Many bacteria swim in liquid or swarm on surface using the flagellum rotated by a motor driven by specific ion flow. The motor consists of the rotor and stator, and the stator converts the energy of ...Many bacteria swim in liquid or swarm on surface using the flagellum rotated by a motor driven by specific ion flow. The motor consists of the rotor and stator, and the stator converts the energy of ion flow to mechanical rotation. However, the ion pathway and the mechanism of stator rotation coupled with specific ion flow are still obscure. Here, we determined the structures of the sodium-driven stator of , namely PomAB, in the presence and absence of sodium ions and the structure with its specific inhibitor, phenamil, by cryo-electron microscopy. The structures and following functional analysis revealed the sodium ion pathway, the mechanism of ion selectivity, and the inhibition mechanism by phenamil. We propose a model of sodium ion flow coupled with the stator rotation based on the structures. This work provides insights into the molecular mechanisms of ion specificity and conversion of the electrochemical potential into mechanical functions. |
リンク | Proc Natl Acad Sci U S A / PubMed:39793043 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 3.12 - 3.43 Å |
| 構造データ | EMDB-60580, PDB-8zyv: EMDB-60581, PDB-8zyw: EMDB-60584, PDB-8zyz: EMDB-60585, PDB-8zz0: EMDB-60636, PDB-9ijm: |
| 化合物 | ![]() ChemComp-NA: ![]() ChemComp-HOH: ![]() PDB-1l2k: |
| 由来 |
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キーワード | MEMBRANE PROTEIN / bacterial flagellar stator unit / sodium ion transport / membrane protein complex / Phenamil |
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vibrio alginolyticus (バクテリア)
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