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TitleStructural insight into the Arabidopsis vacuolar anion channel ALMT9 shows clade specificity.
Journal, issue, pagesCell Rep, Vol. 43, Issue 9, Page 114731, Year 2024
Publish dateSep 24, 2024
AuthorsDandan Qian / Yaru Chai / Weiping Li / Bin Cui / Shaoquan Lin / Zhibin Wang / Chongyuan Wang / Le Qing Qu / Deshun Gong /
PubMed AbstractThe Arabidopsis thaliana aluminum-activated malate transporter 9 (AtALMT9) functions as a vacuolar chloride channel that regulates the stomatal aperture. Here, we present the cryoelectron microscopy ...The Arabidopsis thaliana aluminum-activated malate transporter 9 (AtALMT9) functions as a vacuolar chloride channel that regulates the stomatal aperture. Here, we present the cryoelectron microscopy (cryo-EM) structures of AtALMT9 in three distinct states. AtALMT9 forms a dimer, and the pore is lined with four positively charged rings. The apo-AtALMT9 state shows a putative endogenous citrate obstructing the pore, where two W120 constriction residues enclose a gate with a pore radius of approximately 1.8 Å, representing an open state. Interestingly, channel closure is solely controlled by W120. Compared to wild-type plants, the W120A mutant exhibits more sensitivity to drought stress and is unable to restore the visual phenotype on leaves upon water recovery, reflecting persistent stomatal opening. Furthermore, notable variations are noted in channel gating and substrate recognition of Glycine max ALMT12, AtALMT9, and AtALMT1. In summary, our investigation enhances comprehension of the interplay between structure and function within the ALMT family.
External linksCell Rep / PubMed:39269901
MethodsEM (single particle)
Resolution3.34 - 3.8 Å
Structure data

34828

8hiw

EMDB-34828, PDB-8hiw:
AtALMT9 in the apo state
Method: EM (single particle) / Resolution: 3.34 Å

34829

8hiy

EMDB-34829, PDB-8hiy:
AtALMT9 plus malate
Method: EM (single particle) / Resolution: 3.8 Å

60510

8zvf

EMDB-60510, PDB-8zvf:
AtALMT9 plus high malate in low pH
Method: EM (single particle) / Resolution: 3.59 Å

Chemicals

ChemComp-CIT:
CITRIC ACID

ChemComp-PX2:
1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE

Source
  • arabidopsis thaliana (thale cress)
KeywordsTRANSPORT PROTEIN / CHANNEL

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