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TitleThe structural insight into the functional modulation of human anion exchanger 3.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 6134, Year 2024
Publish dateJul 20, 2024
AuthorsLiyan Jian / Qing Zhang / Deqiang Yao / Qian Wang / Moxin Chen / Ying Xia / Shaobai Li / Yafeng Shen / Mi Cao / An Qin / Lin Li / Yu Cao /
PubMed AbstractAnion exchanger 3 (AE3) is pivotal in regulating intracellular pH across excitable tissues, yet its structural intricacies and functional dynamics remain underexplored compared to other anion ...Anion exchanger 3 (AE3) is pivotal in regulating intracellular pH across excitable tissues, yet its structural intricacies and functional dynamics remain underexplored compared to other anion exchangers. This study unveils the structural insights into human AE3, including the cryo-electron microscopy structures for AE3 transmembrane domains (TMD) and a chimera combining AE3 N-terminal domain (NTD) with AE2 TMD (hAE32). Our analyzes reveal a substrate binding site, an NTD-TMD interlock mechanism, and a preference for an outward-facing conformation. Unlike AE2, which has more robust acid-loading capabilities, AE3's structure, including a less stable inward-facing conformation due to missing key NTD-TMD interactions, contributes to its moderated pH-modulating activity and increased sensitivity to the inhibitor DIDS. These structural differences underline AE3's distinct functional roles in specific tissues and underscore the complex interplay between structural dynamics and functional specificity within the anion exchanger family, enhancing our understanding of the physiological and pathological roles of the anion exchanger family.
External linksNat Commun / PubMed:39033175 / PubMed Central
MethodsEM (single particle)
Resolution2.73 - 3.35 Å
Structure data

39034

8y85

EMDB-39034, PDB-8y85:
Human AE3 with NaHCO3- and DIDS
Method: EM (single particle) / Resolution: 2.73 Å

39035

8y86

EMDB-39035, PDB-8y86:
Human AE3 with NaHCO3-
Method: EM (single particle) / Resolution: 2.75 Å

39050

8y8k

EMDB-39050, PDB-8y8k:
The structure of hAE3
Method: EM (single particle) / Resolution: 2.89 Å

60225

8zle

EMDB-60225, PDB-8zle:
hAE3NTD2TMD with PT5,CLR, and Y01
Method: EM (single particle) / Resolution: 3.35 Å

Chemicals

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM

ChemComp-4KU:
2,2'-ethane-1,2-diylbis{5-[(sulfanylmethyl)amino]benzenesulfonic acid}

ChemComp-CLR:
CHOLESTEROL

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

ChemComp-PT5:
[(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho / phospholipid*YM

Source
  • homo sapiens (human)
KeywordsTRANSPORT PROTEIN/INHIBITOR / TRANSPORT PROTEIN-INHIBITOR complex / TRANSPORT PROTEIN / bicarbonate

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