+Open data
-Basic information
Entry | Database: PDB / ID: 8y85 | |||||||||
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Title | Human AE3 with NaHCO3- and DIDS | |||||||||
Components | Anion exchange protein 3 | |||||||||
Keywords | TRANSPORT PROTEIN/INHIBITOR / TRANSPORT PROTEIN-INHIBITOR complex | |||||||||
Function / homology | Function and homology information pH reduction / cardiac muscle cell action potential / Bicarbonate transporters / cardiac conduction / chloride:bicarbonate antiporter activity / solute:inorganic anion antiporter activity / bicarbonate transport / bicarbonate transmembrane transporter activity / regulation of cardiac muscle cell action potential / transmembrane transporter activity ...pH reduction / cardiac muscle cell action potential / Bicarbonate transporters / cardiac conduction / chloride:bicarbonate antiporter activity / solute:inorganic anion antiporter activity / bicarbonate transport / bicarbonate transmembrane transporter activity / regulation of cardiac muscle cell action potential / transmembrane transporter activity / transport across blood-brain barrier / regulation of intracellular pH / transmembrane transport / external side of plasma membrane / membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.73 Å | |||||||||
Authors | Jian, L. / Zhang, Q. / Yao, D. / Wang, Q. / Xia, Y. / Qin, A. / Cao, Y. | |||||||||
Funding support | China, 2items
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Citation | Journal: Nat Commun / Year: 2024 Title: The structural insight into the functional modulation of human anion exchanger 3 Authors: Jian, L. / Zhang, Q. / Yao, D. / Wang, Q. / Chen, M. / Xia, Y. / Li, S. / Shen, Y. / Cao, M. / Qin, A. / Li, L. / Cao, Y. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8y85.cif.gz | 233.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8y85.ent.gz | 171.3 KB | Display | PDB format |
PDBx/mmJSON format | 8y85.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8y85_validation.pdf.gz | 1.3 MB | Display | wwPDB validaton report |
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Full document | 8y85_full_validation.pdf.gz | 1.3 MB | Display | |
Data in XML | 8y85_validation.xml.gz | 42.3 KB | Display | |
Data in CIF | 8y85_validation.cif.gz | 61.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/y8/8y85 ftp://data.pdbj.org/pub/pdb/validation_reports/y8/8y85 | HTTPS FTP |
-Related structure data
Related structure data | 39034MC 8y86C 8y8kC 8zleC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 135961.516 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: AE3 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: P48751 #2: Chemical | #3: Chemical | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Anion exchange protein 3 / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Molecular weight | Value: 0.135 MDa / Experimental value: NO |
Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2600 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3D reconstruction | Resolution: 2.73 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 968201 / Symmetry type: POINT |