EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular basis for the regulation of human phosphorylase kinase by phosphorylation and Ca.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 3020, Year 2025
掲載日	2025年3月28日
著者	Ruifang Ma / Bowen Du / Chen Shi / Lei Wang / Fuxing Zeng / Jie Han / Huiyi Guan / Yong Wang / Kaige Yan /
PubMed 要旨	Phosphorylase kinase (PhK) regulates the degradation of glycogen by integrating diverse signals, providing energy to the organism. Dysfunctional mutations may directly lead to Glycogen Storage ...Phosphorylase kinase (PhK) regulates the degradation of glycogen by integrating diverse signals, providing energy to the organism. Dysfunctional mutations may directly lead to Glycogen Storage Disease type IX (GSD IX), whereas the abnormal expression of PhK is also associated with tumors. Here, we use cryo-electron microscopy (cryo-EM) to resolve its near-atomic structures in the inactive and active states. These structures reveal the interactions and relative locations of the four subunits (αβγδ) within the PhK complex. Phosphorylated α and β subunits induce PhK to present a more compact state, while Ca causes sliding of the δ subunit along the helix of the γ subunit. Both actions synergistically activate PhK by enabling the de-inhibition of the γ subunit. We also identified different binding modes between PhK and its substrate, glycogen phosphorylase (GP), in two distinct states, using cross-linking mass spectrometry (XL-MS). This study provides valuable insights into the regulatory mechanisms of PhK, thereby enhancing our understanding of GSD IX and its implications in tumorigenesis.
リンク	Nat Commun / PubMed:40148320 / PubMed Central
手法	EM (単粒子)
解像度	3.23 - 4.24 Å
構造データ	39775 8z5m EMDB-39775, PDB-8z5m: human phosphorylase kinase alpha/gamma/delta subcomplex - inactive state 手法: EM (単粒子) / 解像度: 3.66 Å 39777 8z5p EMDB-39777, PDB-8z5p: human phosphorylase kinase - inactive state 手法: EM (単粒子) / 解像度: 3.41 Å 39778 8z5q EMDB-39778, PDB-8z5q: human phosphorylase kinase - inactive state 手法: EM (単粒子) / 解像度: 4.24 Å 39779 8z5r EMDB-39779, PDB-8z5r: human phosphorylase kinase alpha/gamma/delta subcomplex - phosphorylation and Ca2+ bound state 手法: EM (単粒子) / 解像度: 3.52 Å 39781 8z5t EMDB-39781, PDB-8z5t: human phosphorylase kinase - phosphorylation and Ca2+ bound state 手法: EM (単粒子) / 解像度: 3.74 Å EMDB-39784: human phosphorylase kinase alpha/beta/gamma/delta subcomplex - inactive state 手法: EM (単粒子) / 解像度: 3.34 Å EMDB-39785: human phosphorylase kinase alpha/beta/gamma/delta subcomplex - phosphorylation and Ca2+ bound state 手法: EM (単粒子) / 解像度: 3.23 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子*YM
由来	homo sapiens (ヒト)
キーワード	CYTOSOLIC PROTEIN / Kinase / glycogenolysis / SIGNALING PROTEIN