Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
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Journal
IF	>30 >20 >10 >5 all

Title	Structures of the mumps virus polymerase complex via cryo-electron microscopy.
Journal, issue, pages	Nat Commun, Vol. 15, Issue 1, Page 4189, Year 2024
Publish date	May 17, 2024
Authors	Tianhao Li / Mingdong Liu / Zhanxi Gu / Xin Su / Yunhui Liu / Jinzhong Lin / Yu Zhang / Qing-Tao Shen /
PubMed Abstract	The viral polymerase complex, comprising the large protein (L) and phosphoprotein (P), is crucial for both genome replication and transcription in non-segmented negative-strand RNA viruses (nsNSVs), ...The viral polymerase complex, comprising the large protein (L) and phosphoprotein (P), is crucial for both genome replication and transcription in non-segmented negative-strand RNA viruses (nsNSVs), while structures corresponding to these activities remain obscure. Here, we resolved two L-P complex conformations from the mumps virus (MuV), a typical member of nsNSVs, via cryogenic-electron microscopy. One conformation presents all five domains of L forming a continuous RNA tunnel to the methyltransferase domain (MTase), preferably as a transcription state. The other conformation has the appendage averaged out, which is inaccessible to MTase. In both conformations, parallel P tetramers are revealed around MuV L, which, together with structures of other nsNSVs, demonstrates the diverse origins of the L-binding X domain of P. Our study links varying structures of nsNSV polymerase complexes with genome replication and transcription and points to a sliding model for polymerase complexes to advance along the RNA templates.
External links	Nat Commun / PubMed:38760379 / PubMed Central
Methods	EM (single particle)
Resolution	2.93 - 3.63 Å
Structure data	35864 8izl EMDB-35864: Structure of the Mumps Virus L Protein Bound by Phosphoprotein Tetramer (composite map) PDB-8izl: Structure of the Mumps Virus L Protein Bound by Phosphoprotein Tetramer Method: EM (single particle) / Resolution: 2.93 Å EMDB-37957: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer Method: EM (single particle) / Resolution: 3.02 Å 37958 8yxl EMDB-37958: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer (Focused map for CD-MTase-CTD) PDB-8yxl: Structure of C-terminal domain of L protein from Mumps virus Method: EM (single particle) / Resolution: 3.13 Å 37959 8yxm EMDB-37959: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer (Focused map for RdRp-PRNTase) PDB-8yxm: Structure of N-terminal domain of L protein bound with Phosphoprotein from Mumps Virus Method: EM (single particle) / Resolution: 2.93 Å 37960 8yxo EMDB-37960: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer (Focused map for tetrameric phosphoproteins) PDB-8yxo: Structure of Phosphoprotein tetramer from mumps virus Method: EM (single particle) / Resolution: 3.49 Å 37961 8yxp EMDB-37961: Cryo-EM map for Mumps Virus L Protein (State 2) Bound by Phosphoprotein Tetramer PDB-8yxp: Structure of mumps virus L protein (state2) Method: EM (single particle) / Resolution: 3.01 Å 37962 8yxr EMDB-37962: Cryo-EM map for Mumps Virus L protein (state2) Bound by Phosphoprotein Tetramer (Focused for tetrameric phosphoprotein) PDB-8yxr: Structure of Phosphoprotein Tetramer from mumps virus Method: EM (single particle) / Resolution: 3.63 Å 37964 8x01 EMDB-37964: Structure of the Mumps Virus L Protein (state2) Bound by Phosphoprotein Tetramer (composite map) PDB-8x01: Structure of the Mumps Virus L Protein (state2) Bound by Phosphoprotein Tetramer Method: EM (single particle) / Resolution: 3.01 Å
Chemicals	ChemComp-ZN: Unknown entry
Source	mumps orthorubulavirus
Keywords	VIRAL PROTEIN / Mumps virus polymerase complex / RNA-dependent RNA synthesis / Large protein / phosphoprotein.