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TitleMolecular principles of the assembly and construction of a carboxysome shell.
Journal, issue, pagesSci Adv, Vol. 10, Issue 48, Page eadr4227, Year 2024
Publish dateNov 29, 2024
AuthorsPeng Wang / Jianxun Li / Tianpei Li / Kang Li / Pei Cing Ng / Saimeng Wang / Vincent Chriscoli / Arnaud Basle / Jon Marles-Wright / Yu-Zhong Zhang / Lu-Ning Liu /
PubMed AbstractIntracellular compartmentalization enhances biological reactions, crucial for cellular function and survival. An example is the carboxysome, a bacterial microcompartment for CO fixation. The ...Intracellular compartmentalization enhances biological reactions, crucial for cellular function and survival. An example is the carboxysome, a bacterial microcompartment for CO fixation. The carboxysome uses a polyhedral protein shell made of hexamers, pentamers, and trimers to encapsulate Rubisco, increasing CO levels near Rubisco to enhance carboxylation. Despite their role in the global carbon cycle, the molecular mechanisms behind carboxysome shell assembly remain unclear. Here, we present a structural characterization of α-carboxysome shells generated from recombinant systems, which contain all shell proteins and the scaffolding protein CsoS2. Atomic-resolution cryo-electron microscopy of the shell assemblies, with a maximal size of 54 nm, unveil diverse assembly interfaces between shell proteins, detailed interactions of CsoS2 with shell proteins to drive shell assembly, and the formation of heterohexamers and heteropentamers by different shell protein paralogs, facilitating the assembly of larger empty shells. Our findings provide mechanistic insights into the construction principles of α-carboxysome shells and the role of CsoS2 in governing α-carboxysome assembly and functionality.
External linksSci Adv / PubMed:39612341 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.8 - 3.04 Å
Structure data

39596

8yvc

EMDB-39596: cryo-EM structure of carboxysomal midi-shell:icosahedral assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T = 19)
PDB-8yvc: Cryo-EM structure of carboxysomal midi-shell:icosahedral assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T = 19)
Method: EM (single particle) / Resolution: 3.04 Å

39597

8yvd

EMDB-39597: cryo-EM structure of carboxysomal midi-shell: icosahedral assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T = 16)
PDB-8yvd: Cryo-EM structure of carboxysomal midi-shell: icosahedral assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T = 16)
Method: EM (single particle) / Resolution: 2.75 Å

39598

8yve

EMDB-39598, PDB-8yve:
cryo-EM structure of carboxysomal midi-shell: icosahedral assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T = 9)
Method: EM (single particle) / Resolution: 2.3 Å

39599

8yvf

EMDB-39599, PDB-8yvf:
cryo-EM structure of carboxysomal midi-shell: assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T=9 Q=12)
Method: EM (single particle) / Resolution: 2.99 Å

39601

8yvi

EMDB-39601: cryo-EM structure of carboxysomal midi-shell: icosahedral assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T = 13)
PDB-8yvi: Cryo-EM structure of carboxysomal midi-shell: icosahedral assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T = 13)
Method: EM (single particle) / Resolution: 2.93 Å

50109

9f0h

EMDB-50109, PDB-9f0h:
cryo-EM structure of carboxysomal mini-shell icosahedral assembly from co-expression of CsoS1C, CsoS4A, and CsoS2-C (T = 9)
Method: EM (single particle) / Resolution: 1.8 Å

PDB-8yxu:
Crystal structure of CsoS1A/B (modeled with CsoS1A)
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

Source
  • halothiobacillus neapolitanus (bacteria)
KeywordsSTRUCTURAL PROTEIN / Carboxysome / shell / Icosahedron / mini-shell / Halothiobacillus neopolitanus

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