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Structure paper

TitleConservation and specialization of the Ycf2-FtsHi chloroplast protein import motor in green algae.
Journal, issue, pagesCell, Vol. 187, Issue 20, Page 5638-5650.e18, Year 2024
Publish dateAug 26, 2024
AuthorsKe Liang / Xiechao Zhan / Yuxin Li / Yi Yang / Yanqiu Xie / Zeyu Jin / Xiaoyan Xu / Wenwen Zhang / Yang Lu / Sheng Zhang / Yilong Zou / Shan Feng / Jianping Wu / Zhen Yan /
PubMed AbstractThe protein import motor in chloroplasts plays a pivotal role in their biogenesis and homeostasis by driving the translocation of preproteins into chloroplasts. While the Ycf2-FtsHi complex serves as ...The protein import motor in chloroplasts plays a pivotal role in their biogenesis and homeostasis by driving the translocation of preproteins into chloroplasts. While the Ycf2-FtsHi complex serves as the import motor in land plants, its evolutionary conservation, specialization, and mechanisms across photosynthetic organisms are largely unexplored. Here, we isolated and determined the cryogenic electron microscopy (cryo-EM) structures of the native Ycf2-FtsHi complex from Chlamydomonas reinhardtii, uncovering a complex composed of up to 19 subunits, including multiple green-algae-specific components. The heterohexameric AAA+ ATPase motor module is tilted, potentially facilitating preprotein handover from the translocon at the inner chloroplast membrane (TIC) complex. Preprotein interacts with Ycf2-FtsHi and enhances its ATPase activity in vitro. Integrating Ycf2-FtsHi and translocon at the outer chloroplast membrane (TOC)-TIC supercomplex structures reveals insights into their physical and functional interplay during preprotein translocation. By comparing these findings with those from land plants, our study establishes a structural foundation for understanding the assembly, function, evolutionary conservation, and diversity of chloroplast protein import motors.
External linksCell / PubMed:39197449
MethodsEM (single particle)
Resolution2.8 - 2.9 Å
Structure data

38589

8xqw

EMDB-38589, PDB-8xqw:
Cryo-EM structure of the Ycf2-FtsHi motor complex from Chlamydomonas reinhardtii in AMPPNP bound state
Method: EM (single particle) / Resolution: 2.9 Å

38590

8xqx

EMDB-38590, PDB-8xqx:
Cryo-EM structure of the Ycf2-FtsHi motor complex from chlamydomonas reinhardtii in apo state
Method: EM (single particle) / Resolution: 2.8 Å

EMDB-38591: Cryo-EM map of the Ycf2-FtsHi motor complex from Chlamydomonas in ATP-bound state
Method: EM (single particle) / Resolution: 2.8 Å

Chemicals

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

ChemComp-DGA:
DIACYL GLYCEROL

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

PDB-1lxl:
NMR STRUCTURE OF BCL-XL, AN INHIBITOR OF PROGRAMMED CELL DEATH, MINIMIZED AVERAGE STRUCTURE

ChemComp-ZN:
Unknown entry

Source
  • chlamydomonas reinhardtii (plant)
KeywordsMEMBRANE PROTEIN / ATP-motor / Ycf2 / FtsHi / Fhl

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