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- EMDB-38589: Cryo-EM structure of the Ycf2-FtsHi motor complex from Chlamydomo... -

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Basic information

Entry
Database: EMDB / ID: EMD-38589
TitleCryo-EM structure of the Ycf2-FtsHi motor complex from Chlamydomonas reinhardtii in AMPPNP bound state
Map data
Sample
  • Complex: The Ycf2-FtsHi motor complex from Chlamydomonas
    • Protein or peptide: x 21 types
  • Ligand: x 9 types
KeywordsATP-motor / Ycf2 / FtsHi / MEMBRANE PROTEIN
Function / homology
Function and homology information


phytochromobilin biosynthetic process / oxidoreductase activity, acting on the CH-CH group of donors, iron-sulfur protein as acceptor / poly(ADP-ribose) glycohydrolase / cobalt ion binding / acyl carrier activity / chloroplast / oxidoreductase activity / hydrolase activity / membrane
Similarity search - Function
Fatty acid desaturase / Acyl carrier protein, chloroplastic / Ferredoxin-dependent bilin reductase / Ferredoxin-dependent bilin reductase / ADP-ribosylation/Crystallin J1 / ADP-ribosylglycohydrolase / ADP-ribosylation/Crystallin J1 superfamily / : / : / Acyl carrier protein (ACP) ...Fatty acid desaturase / Acyl carrier protein, chloroplastic / Ferredoxin-dependent bilin reductase / Ferredoxin-dependent bilin reductase / ADP-ribosylation/Crystallin J1 / ADP-ribosylglycohydrolase / ADP-ribosylation/Crystallin J1 superfamily / : / : / Acyl carrier protein (ACP) / Phosphopantetheine attachment site / ACP-like superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Cell division protein / Uncharacterized protein / Uncharacterized protein / 4Fe-4S ferredoxin-type domain-containing protein / Flagellar associated protein / ADP-ribosylhydrolase ARH3 / Fatty acid desaturase domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein ...Cell division protein / Uncharacterized protein / Uncharacterized protein / 4Fe-4S ferredoxin-type domain-containing protein / Flagellar associated protein / ADP-ribosylhydrolase ARH3 / Fatty acid desaturase domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Acyl carrier protein
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsLiang K / Zhan X / Wu J / Yan Z
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32271239 China
CitationJournal: Cell / Year: 2024
Title: Conservation and specialization of the Ycf2-FtsHi chloroplast protein import motor in green algae.
Authors: Ke Liang / Xiechao Zhan / Yuxin Li / Yi Yang / Yanqiu Xie / Zeyu Jin / Xiaoyan Xu / Wenwen Zhang / Yang Lu / Sheng Zhang / Yilong Zou / Shan Feng / Jianping Wu / Zhen Yan /
Abstract: The protein import motor in chloroplasts plays a pivotal role in their biogenesis and homeostasis by driving the translocation of preproteins into chloroplasts. While the Ycf2-FtsHi complex serves as ...The protein import motor in chloroplasts plays a pivotal role in their biogenesis and homeostasis by driving the translocation of preproteins into chloroplasts. While the Ycf2-FtsHi complex serves as the import motor in land plants, its evolutionary conservation, specialization, and mechanisms across photosynthetic organisms are largely unexplored. Here, we isolated and determined the cryogenic electron microscopy (cryo-EM) structures of the native Ycf2-FtsHi complex from Chlamydomonas reinhardtii, uncovering a complex composed of up to 19 subunits, including multiple green-algae-specific components. The heterohexameric AAA+ ATPase motor module is tilted, potentially facilitating preprotein handover from the translocon at the inner chloroplast membrane (TIC) complex. Preprotein interacts with Ycf2-FtsHi and enhances its ATPase activity in vitro. Integrating Ycf2-FtsHi and translocon at the outer chloroplast membrane (TOC)-TIC supercomplex structures reveals insights into their physical and functional interplay during preprotein translocation. By comparing these findings with those from land plants, our study establishes a structural foundation for understanding the assembly, function, evolutionary conservation, and diversity of chloroplast protein import motors.
History
DepositionJan 10, 2024-
Header (metadata) releaseSep 11, 2024-
Map releaseSep 11, 2024-
UpdateNov 20, 2024-
Current statusNov 20, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_38589.png

Downloads & links

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Map

FileDownload / File: emd_38589.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.09 Å/pix.
x 360 pix.
= 391.32 Å		1.09 Å/pix.
x 360 pix.
= 391.32 Å		1.09 Å/pix.
x 360 pix.
= 391.32 Å

Surface

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Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.087 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.45
Minimum - Maximum-1.9895936 - 4.162306
Average (Standard dev.)0.0048152357 (±0.1308622)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 391.32 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_38589_half_map_1.map
Projections & Slices
AxesZYX

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Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_38589_half_map_2.map
Projections & Slices
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Sample components

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Entire : The Ycf2-FtsHi motor complex from Chlamydomonas

EntireName: The Ycf2-FtsHi motor complex from Chlamydomonas
Components
  • Complex: The Ycf2-FtsHi motor complex from Chlamydomonas
    • Protein or peptide: Fhl1
    • Protein or peptide: Fhl3
    • Protein or peptide: Ycf2
    • Protein or peptide: Ctap1
    • Protein or peptide: Ctap6
    • Protein or peptide: ARHL
    • Protein or peptide: PcyA
    • Protein or peptide: CrTam39
    • Protein or peptide: ACP
    • Protein or peptide: CrTam29
    • Protein or peptide: CrTam34
    • Protein or peptide: FADL
    • Protein or peptide: CrTam15
    • Protein or peptide: CrTam49
    • Protein or peptide: Ctap7
    • Protein or peptide: Tic22
    • Protein or peptide: DnaJ
    • Protein or peptide: CrTam35
    • Protein or peptide: UNK
    • Protein or peptide: UNK
    • Protein or peptide: CrTam31
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
  • Ligand: MAGNESIUM ION
  • Ligand: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
  • Ligand: DIACYL GLYCEROL
  • Ligand: CHOLESTEROL HEMISUCCINATE
  • Ligand: Beta-Sitosterol
  • Ligand: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
  • Ligand: ZINC ION

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Supramolecule #1: The Ycf2-FtsHi motor complex from Chlamydomonas

SupramoleculeName: The Ycf2-FtsHi motor complex from Chlamydomonas / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#18, #21, #20, #19
Source (natural)Organism: Chlamydomonas reinhardtii (plant)

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Macromolecule #1: Fhl1

MacromoleculeName: Fhl1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 128.932844 KDa
SequenceString: MQAAMHAQRP AGPPCSSAPS TSYPVAPSPV SSRSRGLHAR RGVAEQRSLG CRSTGSSDQH SNTNDGASGP SRPEQGPELD WSGLPRRQL AAMAMSPFAA LSLPLVNDPA WQQSFETYGG KLREVLLGQQ EAAKNVAKQL DEGVTYMDWT YRSTGVDLSA V WDPELWIR ...String:
MQAAMHAQRP AGPPCSSAPS TSYPVAPSPV SSRSRGLHAR RGVAEQRSLG CRSTGSSDQH SNTNDGASGP SRPEQGPELD WSGLPRRQL AAMAMSPFAA LSLPLVNDPA WQQSFETYGG KLREVLLGQQ EAAKNVAKQL DEGVTYMDWT YRSTGVDLSA V WDPELWIR FREAVAQNEP AIFWNKLLDR VQYKENLPQA GLVGDMRISY AKFLELLKDQ RVKRLVVYGD MRTAVVEVPH PW SASVLGH PATHPFYEDS AHNRVSMLRP NPAAPEDVTQ WFCAEMPEWD MEKYRFYVDL PGDFWESGVL QRHLAAQRAE GAV WDPASG QYILPYRAQK KVFQVSTEVQ LLDPQESWDF LGWLLAPGRL EFYEKAACVA IALRVLGIVI AISTGSPLFK LVNV GWGKL RGKGKKNATK DPKKMSKQEK KESQWERLTS SRAREFMTKD EKTGKMRDTG VRFEDIAGME FLVTEMREIV RMLKG DEAY KRVGAKCPKG IIFQGPPGTG KTYLARAIAG EAEVPFFSSV GSEFVEMFAG VAAARVNSLF YNARKKAPAI IFIDEI DAI GRARSTLGGD PGSMERESAL LAMLVQMDGI ANKTEQVLTI GATNLAQELD AALLRPGRFE VVYEVPQPGP SARMAIL RY HAKGKPLEGD GQRLLLKTAE ATQGWSAAAL ANLMNEAAIL TVRRNVPAIS LPMVLELVEG LNWGEQAPRI PDSEAKDR L ALITAAKAVA FALTPGLEPI KSVTMWSGRR GLGPSVDFIA MEDKAAMDMH PEETELMGWR TNFKTNAAVV GDEPLGEFA HVAGLLVPLY AGRAAEVALF GKDGASLATA QPLADCFEIA YYCVRNSQVH PRFKSLPPLH TTMWLGRDDA GRWRRDPLAI GFDEELGYH KLTLTLLKAS WRRALRLVAQ RRSAITKVAA EMLAAPEEKI TGARLVEIIE STPLDDLGGE GLDGAAAAAV V EEAGNEFL PLLKEVLGQV PGIILTGESL AQTDDQGRPL PPSSASTSSA DAAASDAAAS AGPATELRLD DATLAAVSRT LM GRLDVVD LIGRNTAVEA AERVRDALLH PETRERLLAM RRWVEGGPGA PEFPPSPLSP EQTAAMSPSG PLYGNLALNL DWW RRRQDN VISWSAMEIL MSRRQVDLYK QDADMTEGAI AKLGPPPAAP AAAIGSSSKS SSGQSS

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Macromolecule #2: Fhl3

MacromoleculeName: Fhl3 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 120.645609 KDa
SequenceString: MRMSGMAIRC AASGSLLASP AASSRPAWRA APVLCSPRVP CTPLELGVSC RRSCMQRRWS RAANVRTLAT SRGEQPQDSG PSTSGRAEL PLDSGIGKLI STTAKAIGLV GLMAVAVLSG PTRAAHARDR LSAQPAAEAL IHHQQPYQQP HHHQQQHRSA G AVANPVLS ...String:
MRMSGMAIRC AASGSLLASP AASSRPAWRA APVLCSPRVP CTPLELGVSC RRSCMQRRWS RAANVRTLAT SRGEQPQDSG PSTSGRAEL PLDSGIGKLI STTAKAIGLV GLMAVAVLSG PTRAAHARDR LSAQPAAEAL IHHQQPYQQP HHHQQQHRSA G AVANPVLS DLAAAPATLE PATLEPATST TSALTPVEAA YSAYLRRIAE AYLAEHPQMA APEHAAHVAR VVRSRALGTP LS FDELMRS AVPAPGEVPN RNSRGQVAEQ VRAILDQYDR EDFDLGIKQF MLEAKVKAKL EAASRGTSRD RAAPKDYEEA LAA ELFAAE EGAAPKEKAK (TPO)EDMVDDAF(TPO) (TPO)EVVEEAMAL FGDANSVKTA WRTQEVLREL SYTQLWALVG EG HVARVRF YGPEKNKVMA TTRASAPGGE RLCKVVLPPD PELLDHLVSN GVVVDTGVTE DDRLRASLLV QMLRYTVPFM VIS GLFWMI HTWILDPLPN KFRRQEFIRY RREMLHVASK LNFRTPAREV RIDTGSPDFI KWDDINGIDE VKKEINEIIE YLRN PALLR SRGVARIGGV LLAGAPGTGK TLLAKAIAAE GGVRMFTCSG TDFYDVYSGV GARRVRETFD RLRNAAPAIL FIDEF DAMG AARGAQASGD ESASIINELL VQMDGFEDNR GIVVLGATNR PGAIDSALIR PGRFDRIIYM PLPDALGRAK IMQVHA RNK AVDPNINWYE VARAMAGFTG ADVMGLMARA ARMAARQGRH AITEDDIYAA MENKTMEATL EASTAGDGGG LVGGEGV EG SPDPIPPQLR RAVSVYEAGK ALLAYITPDY EEIARVSVCP LNVLTGFTLF VEDEDKNVNA ILTRSELEGR MVVHLAGR C AEKLVMGEGQ MTGMGSPDLF HANLIAREMI MSMGMGRRTG PIDLLRVAAT SEAASGADTL RAGPAAADGD PFYYHTTDM STEQARVALA EVVELLDAAE AKAMYGLAIN WRALQALTQA LLDRGTITGK EVAHILESNG VIHFPDPYTT GFGWDPDGSL RYPFKPDTP PEGGSGGGGA AAEGSAPQTP DLSGARGKTW FAGTAYDAPR NADGTFKHGW HWNMPFSVKT ELPDWYKKEV E RYSY

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Macromolecule #3: Ycf2

MacromoleculeName: Ycf2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 342.188906 KDa
SequenceString: MTFLNHYTYL FSIPEKQADK VSGILRLAQA RPIETLQNER INKQLNAFLK TYKFEKLITN YKKMQSFIPN NSLNGNKTNS STNKLYATS LNVFPENPPL MVRKAVSDEA DKFSKFTYSK VQVVTNNLNN GMNSKEFIKA NNLKPSLRAA ESLVLNHLTY N KFKENLYF ...String:
MTFLNHYTYL FSIPEKQADK VSGILRLAQA RPIETLQNER INKQLNAFLK TYKFEKLITN YKKMQSFIPN NSLNGNKTNS STNKLYATS LNVFPENPPL MVRKAVSDEA DKFSKFTYSK VQVVTNNLNN GMNSKEFIKA NNLKPSLRAA ESLVLNHLTY N KFKENLYF KTNNIQPTKS KSTSLFFLNI LSNSKPRTCS DFLSSPKIRK TWFRNTAWSL QTQQHRSSNG INLSLQLPYA LG PSVPAGA SGQNMYELPV AQSSSRFGTY YFLQKLLSKY LDVWNASADN GSVLSNSENI KLNFSMVSLL DSKMAIQTPN SLY FVFTQL NQKTFLSYWL LPVAGLALLT PTLLTLTGQS VSVQKFNSFI NKKTDMMVLS NTEMPSKSFG TPTLFGTSVE IYLP NSYMP KGEGESGINR VNSSINAVKK NTVTANLVLD SESQEVATSF QNDLISIKYC FNNLYNYISN KTALSTKNLF LFSAI KSNA TKHKRTQSFF SVENTTTLGN NSNFVKGHFK SSINAFSSYL PSTNVHSMIP LTSLPYLKAI SPLYSKFMID HSLKFI TPK TTLKLLQHKL NKSPKQMYTK TQNFTGLRDL RALNSFSFGQ VNFRTNHFLH SNSRPLNHYN QALKLINGYE QYKNNLQ IN CNKTLDLNTK NKLVYQVNKS HLFNQKCSQI VYKQSLYNRD LCTIRGTGTK VVDYFSHGDK LSNKNGIVLD YFVYSNLL F DNKTNTIINK DGKQNITKLK LNLTKTTVPF KTLIKKYTSI NSLVANEQTR NNLNLGLIHF NGHLSVVSNA NLLTGRPVK FIYYKFDKRL NSYLIYVNQN LKKFIQLNNN FLKPKPLSHQ KNKPVEDFNQ YATNNSSPPK TNVFEKSFVE DSSLRKPLTS LRGSKQFLN SLTILFKHQK MFKKKTLKAH KWHSDTQGIF RKHTNSSFGS ANFSNGPEES SLSTRLHIQK KRKAKKQRLE T RRQKKRTR FFPRPVWLRS RMFLNFLTER NKYYLNSTIT KQGFSLPSKD VVTTKLDWLK EDMRRLPLGA YQYKSLLTQK AG NKFQRQS FTEVVSTMEY INGIHKALNN SIFNKIVRKS LLSSSQNPLK LRLVANYSKM QFMHRVKLPF YRTLKHSEGT KNL ANKKQN LRDIKIKANY NNFKSQKANN QPQQNDKDKD KDTMFRDFWV WSYNNTQTNA FNQNLWWLLP NLTTKQSNLE FLTS TYPTA KETQRAKEEI HGNSIPTASK NQIALIRLNW ALNKTNINTF TDYSKRNNLW TTQKLRNQSK NNKTKSLEKQ FITNW EKFF LNKNLNIFSK KIISKVKQKK QKLNYMTSYL NVQSEHNVKI FHNSWWTHLN IKNLVNNQDM VIPVREGYFS VGNFNS EFI NSAIIKSINN KTLVENYVYS PSSEKETMQL LLMSSSILLH LCAIISLVSI SQVRCFVKFH LILLYKLSNV YNAILNQ LS NKLQKNLPIY NNINKLNSRY FYMNHQKSQI KQRKKLLTYF SLTLLKKQFV TVKPLQIRNF ASIKNQSSNN SNLTYTDM L PLSLRANKFR GSKYDISIRE EEGQSAHIKP SKSMYAKLNI LSLKTIFLKQ LLMNKKPSAL PSNVGLKSNR ETQKSQLIQ RIKTKELQIS LKKNIIGFSK VTKNHILKIL FNVIEVFQTA VRNISSFFEK PAEFTTTWIA YGFLVEWSSD FITIIPENVD IYIWNVFSK IYRTIPLSFI STTLGPASTV FDPVTNSTIP IQMGNFNYQK MVAFPILLSL SHLLHRRILY LFDTLFSTIT Q PDTDLIAR QEKGTLFWDI WADFLVTAAD YYNVNVAALS TIKAEQNSLI ENISNDFDNL TMSSKKPFFM PNKGVSNIKN IF WIKKLKE PQLPESIVQN REVFVRERKR TLKGLFNIYA PQEETLWNNP TSPKNLSDEK ISFKLFNQLN LQLFAEKNKI KPY FEAYFS TTQQKTNIMQ SAFPEANLNR WSVNQFITYQ SWHSHNGSNN SNGDLFIDYH PPKTFSHIPA LKYNSILQQP IGSL VCQIY SGLFNKQISK NILLVNPKTT SNNLVDYNVL LIQALAGETE MKIITDNAQR YALVNRGFAI GIKLLREVFD AIALN TPCI FLLEDIHAIG ERRPMLISDF GGGMSDDNGS FKEDFFGSQR DEVHEKNQVV YQLTRHAITH YKKPFKGDYS LAIPTN LYV TDLFLKLPTQ SISNLTNVEN HNLSIKNKIQ HNGTQSLTET KRNLGGDINK NSYLQLTQFT KTLAPPSTSP FSVLLLK EE KRLKPNKIVE ELPWTSLPGE QLATKPRTSY SVRAKVAMLA ELSLSNLSAK LDMITDLLVI IDSVRSNKGF VVFATTDI P HVLDPALRRP GRLDETICLP NIHTSNILNF TKNYEIFKSA KDTSNFGKKI ILNEMQNLTT TSTQRDMYLS CLPTNNQTH KTKREGVLTM NLKDYNILLN QVYFAEGTGG ILNSQMHKDS LQKSLNFALI SHSKKLKELN VSKLIGSNGT VSQGNVDQLG VFAGQIVNK QKKSLQQHLP NSKKSFKKKY KDKAIIYYEV GKFVLNYFLN NQLTQSSIID KPVSVTNKQT NDITIFGNDF L NLKTINYL SLYNSKNKIL LQLMLIFGGK ISQLLSSKNL VKSLKQASIN SYMVEEESGS ISSAGMPLGQ THLLPKALSV LA KPMIFSD GYNNQNLKTA TTLLLSFIHK RYLYRKNLIV PKLLSFADGN ILDEPPSPPF SSLLIPAKRF ENYKRFFRDT LTG DKMGQR KSQITLLEKL QYHMQLRSIK QLNATFSSQE NLDFQSNAAL TSQKLDTLMS LSTNNLLQNP TNINWYYQNR ILKR HGQYL TNQWWNGQLS EHNAETVFLS DIDWRSSFIK NKNINITKSK NLYRLTQQKN NTDGLDVLLD FPDTDQYYNP KRRRW LLNN GSWNFWFNFD KLYSEEIVTT WILESLIQTY KYLHKNTELL DFVTNKFITL GYIAPENANL QNISGFPSQS ELLSTK EII LTNSFKRF

UniProtKB: Cell division protein

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Macromolecule #4: Ctap1

MacromoleculeName: Ctap1 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 107.778039 KDa
SequenceString: MRSAELGRPP RLAQSRLRNV SSHHVTNSCL WLRPPGCRRL VASCAASKES SSASLTAERF ITDAKELNAT GSGLPIIDGP DWEEQHWAA LKAMSAGRPV ALPTPHAKFG PEDLQRIAAS GPRLEDLTLE HAERLAGPGQ LPTAPDGVAL AFRYIPRSVL G DFRQEVEP ...String:
MRSAELGRPP RLAQSRLRNV SSHHVTNSCL WLRPPGCRRL VASCAASKES SSASLTAERF ITDAKELNAT GSGLPIIDGP DWEEQHWAA LKAMSAGRPV ALPTPHAKFG PEDLQRIAAS GPRLEDLTLE HAERLAGPGQ LPTAPDGVAL AFRYIPRSVL G DFRQEVEP DWRSLPAMSP AELYAGLRAR NWTSAHYDPA AEPWRLQVFS CDYKHTGVTG WPGYRVVVTS RGGRRRWVDL AE EGELVQL TEQAPPASPA DIGYSHVFAQ LYQAYEPRYS PEALAALYGS SSSKGKAAAA AAAQHDTPAL RHLDVSYHGT GSA VAPGSG TAFLMQPSWD AVTGAIRWGL ERSGLPELRA LRDSLLPEWR PPALELNRSN NNLGVVYFAV CLTLGIVIPA LRRS RILDI RTLEEDPGAA MEFARSKSEA RKEGLTGVEF RDVAGLGPIL NEVVEVVEFL KDPGTFSKLG ARPPKGILLE GDPGT GKTL LAKALAGEAM VPFYQMSGTE FTEGIVGLGA ARVRDLFKRA RATAPCVIFV DEIDALGLRR AENDSAKTNE EREQTL NQL LTEMDGFTPD TGVVFLGATN RADLLDPALM RPGRFDRKIR MPKPDTEGRL EILKLHLRNK QVAPDVDLLQ LARDLPG LV GADLANIVNE AAMTAVRSGR QQLTARDIYA GVDRFTQGEV RPSLPTAHKL PVLCFAAKEI GIALVAGELR DRYGRVEL V ERVSIQPKGR AYSRTMFQRG TDEEYQLMTR GRLLDRIRLA LAGGFAVRTA LGEETNFTAA DIKRATRMAK KYVFYYGFS EAGGAGITTW ANQPYSGDFV IGQQRARKVV STDAMDAFAD WPTVSEDFRF DAPSPSDVTW HRYTDEVRRV LKGCSEDVLG ILAERQEAM WAGIKALSDR KELLGSELRD IFDAHPAATS RDRDARAELA AAKLDMTIFT EGANSRWPYG IEWLDDAYPK P YWVQQQEA EAAEAQAKQP AAAL

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Macromolecule #5: Ctap6

MacromoleculeName: Ctap6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 109.355469 KDa
SequenceString: MKATGLPSLP ARALGAAGCS TSPRPAALGW SSRGCASGRR RACARVHVAD AEAVASGVAA TEAAAAVPAL PARATAVVAP LPEKNYGSL RGGRWPFLYD NVYGLPVVRQ VASYGEVLEG IRTGRISQVL WFQAPRAVTA SAAAPPPGLG GPQQPQPPPL A SPDGRCLV ...String:
MKATGLPSLP ARALGAAGCS TSPRPAALGW SSRGCASGRR RACARVHVAD AEAVASGVAA TEAAAAVPAL PARATAVVAP LPEKNYGSL RGGRWPFLYD NVYGLPVVRQ VASYGEVLEG IRTGRISQVL WFQAPRAVTA SAAAPPPGLG GPQQPQPPPL A SPDGRCLV RFANGQVKQA VIPPGEPRIS QALQQYGTAV SYIPLEPRYM PELAAMRARG AQEAVLGEVD TGAVATPVEL PE DERRGAA VGPTAFEAVA AYGSPEQLAA ALDDNYQAAA GQVAALLAER EAWVAEQEAL EAAARAERSM SDRAGGGGGG GGT ALVPSG GFSVGAWLDS IQLTNEQQAM VLKYVPILGP ILGSGFIIGL YLLARLVKGD LTDRLKMMDS EADKKKKTAL KEAR IAFLE EEVPGLVAKG ASLDDVRKRV QPVNARLGTK LAIGDGEIQS TYEACRLLLS EGVDLSAASS TAASGALAQM ESDER RAAA GAAEGGGEGG DAMNAMMEMG KLNTARIRKA TDPKIMDVKK RVRDVRRKLK RESKVQLSDE IIFFDDIAGN KQAKVE LME VVDFFRTPEK FKASGARAPK GVLLVGPPGN GKTLMARAVA GESGVAFISS SAAEFIEMYM GLGAARVRDL FNTARSV AP CIIFIDELDA VGRQRQGGGR SNDERDNTVN QLLTEMDGFE AEQQGIVVMG ATNRKDVLDA ALTRPGRFDR SIEVRRPD F QGRLEAVKVH LRDKPVAAEI DYVSLASLMG GMSGAQIAGV ANTACFLASR DGRSEVNQTD LTLAVEQAKY GRAYDQSRF VGAGRKKRFA VMEASIALAA TLLPAIEPVE YATIIPSTRS PLGRTVLKPH VGRYTTGVWT YRYLREQLLV ALAGRAGEEL VLGRDELSS LNQHRLQMAR QVAWKIMNSG MSSHPDYQHL RGLGSNYFDG SSEPGRFQQT TVVMDANQTR SEAVDADMEV E GLLNGGYK QVFELLVRNR AALDALTELL LEREKISGEE VVQVVEELGH PEDLARRAQW AGYELL

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Macromolecule #6: ARHL

MacromoleculeName: ARHL / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 51.410547 KDa
SequenceString: MRQSVRSPGT RASLQSGRAA SSCLPVTPLC PLVASTTSAP ATSISSSDAL LRSTAVASTS TSSPATVPIA ARRRHQPRSR SVAAATPAA APSPAAAAAP AWRGPGEVGD EDKAVGCLLG AAVGNVLAAP YQGDRHFEVI RLRRNGVTDF WKYDIGAQPV Q YGQYTGDF ...String:
MRQSVRSPGT RASLQSGRAA SSCLPVTPLC PLVASTTSAP ATSISSSDAL LRSTAVASTS TSSPATVPIA ARRRHQPRSR SVAAATPAA APSPAAAAAP AWRGPGEVGD EDKAVGCLLG AAVGNVLAAP YQGDRHFEVI RLRRNGVTDF WKYDIGAQPV Q YGQYTGDF ANLLAVATSL SASRGVEPAH LLGALTRAYA EGGSSVEVED ASGGSSSGFL PARRYSPYDR LVMDAVLAGT DP LKVPELA ERYLAETTRR HASSSSDRPD REPHGPSDLG AAARAAPIGL AYRRAGGERL LAAVRRSLEF SHPTPLGLDA AHV VAAAAA WCGRQQPGDA VGATPAALLS HLLNDVAVTA EQCGKLRLLR DNLFQLDEVT DWRAFYAGPQ WARLTALFSR LSFH GLATA GSEFASVVLL ALLSSWGRPE QAVIVAASLG GHAPATAQTV GALAGTLYGQ SWVPERWWRG LGEGVEGEAG REAVV QAGR ALAAVELADG L

UniProtKB: ADP-ribosylhydrolase ARH3

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Macromolecule #7: PcyA

MacromoleculeName: PcyA / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 61.329266 KDa
SequenceString: MMSSIPKSIG AQRSAASTRA HALARPVVLA PAASIPARSQ GVTSTSGRCL APPPRAAAGA GAPGTAGPTN AGAAAHEVEV DAVESPLSP EDIMRLVQQH EDVAAAAESE QLVAQFRDDP QGLYEYVNRA YAEGPRRVTT PISLLQEEIT GAVTESYPAA V ANDIIGMG ...String:
MMSSIPKSIG AQRSAASTRA HALARPVVLA PAASIPARSQ GVTSTSGRCL APPPRAAAGA GAPGTAGPTN AGAAAHEVEV DAVESPLSP EDIMRLVQQH EDVAAAAESE QLVAQFRDDP QGLYEYVNRA YAEGPRRVTT PISLLQEEIT GAVTESYPAA V ANDIIGMG SWRLKDDVDP VIEFLVARLE GCWREILDTD LCLYPREKWK EQGWDLVDSM DPHQELEGFS YADIPDPAKG EA GYPRLQL ENRVYCSKVF RKLHVEVGLR QDGLQVLHVV VYPRYSYDMP IFGMDIVMVD GRVTLAVVDC CPVRADLKLQ PHY METMAL LQRTFLEGTD PALRRIPEWG SKIFSPLALC ITPSGPEELA AFAKYAVALH RAYLTMSLNA VPVVAGPGDR REAA RLQEI QDGQKRFCDN QLVNKKTRRV LEVAMGVEWT EAYMSQLMFD FDPKYEPPYF DASFEKLYTY FDENPSFGEM ADEAM ELER GAEAERANET MAAALSGRSV SREKLAMAMG FLFQNDATFR AAVQTLSGGQ VDGNIEERLT DDLMQLLERS E

UniProtKB: Uncharacterized protein

+
Macromolecule #8: CrTam39

MacromoleculeName: CrTam39 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 39.328336 KDa
SequenceString: MSPGALYQLA PERLRASSRR SAAATIFRRD RMRLLRCSAA AQPGEPGEAA GPSTSGSDNS NWWASINRKT GIRGPDPAPA EEHTNGPAR DIIGDRMSRR LEDINKAERQ RVWDAMRVAA AHRYASGQMP AWFDPEWLQQ EEAPLNAMDR MRGEQRRIEE Q QQQQGEAS ...String:
MSPGALYQLA PERLRASSRR SAAATIFRRD RMRLLRCSAA AQPGEPGEAA GPSTSGSDNS NWWASINRKT GIRGPDPAPA EEHTNGPAR DIIGDRMSRR LEDINKAERQ RVWDAMRVAA AHRYASGQMP AWFDPEWLQQ EEAPLNAMDR MRGEQRRIEE Q QQQQGEAS SSDKLAMEGG GGDSGAGAGG WSGGGSGGGW WREDDPYWPL RDWGDHPMRW WTLAFAAIMA AGGLATSVAT GY VEPVQAG LGAGALLALA GAAMSDARCV PGALGVKLAW AVCALIVLKE VSVGWQHKRK RRLAASAPRL ELTGLAAAAL CAG YMLTDM SGMGEVALPP NPGAVFKSPD VAYRASVWQK WGYGQVQMRV

+
Macromolecule #9: ACP

MacromoleculeName: ACP / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 12.779737 KDa
SequenceString:
MALSMIRKSA AAPVRRAAAA PVVVRGRRVI TFAAVDKAKV LEDVRSIIST QLGTELEKVA PEAKFVDLGA D(4HH)LDTV EIM MALEEKFEIA LEEEGAEKIA TVQDAADMIA AQIAAKGN

UniProtKB: Acyl carrier protein

+
Macromolecule #10: CrTam29

MacromoleculeName: CrTam29 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 29.084951 KDa
SequenceString: MRPCPAVHRR VPPGPVAGHK QLLPLQRSCR GAPLVCRTAA PMAPSWAETR SAAASAPILE PDMLPSTSAP FISPMTPYVP EEEPTRTPP SIKDTGTLRP ASEWYPQWMQ YRRREDNYVF WQDKFMRCST DIPWAEKRWT LFSTVWYLVQ QLRFVGTPPA L RYVAFLGW ...String:
MRPCPAVHRR VPPGPVAGHK QLLPLQRSCR GAPLVCRTAA PMAPSWAETR SAAASAPILE PDMLPSTSAP FISPMTPYVP EEEPTRTPP SIKDTGTLRP ASEWYPQWMQ YRRREDNYVF WQDKFMRCST DIPWAEKRWT LFSTVWYLVQ QLRFVGTPPA L RYVAFLGW RALMFQVYAA HKALVLWQCK LDAGLARIGS GGATATFSKT MALRRLHWRN SPLAEALYAL NLYKTGRVHL LP PVAKPIP RPTFFWLF

UniProtKB: Uncharacterized protein

+
Macromolecule #11: CrTam34

MacromoleculeName: CrTam34 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 34.185254 KDa
SequenceString: MQSAARGCHA PAGLSSSLLR SRTYVPPCAP SSSGRVVGSR HITARAAGGK GGGKDDKAKE KADFSALWAL RIKNFFSSRR KYLQQAEQV GDDDKEKAFK AEIAKEERSI RELKDELVAI SMEEIEEQEK QGDPRPALAA TDIAQARMDL QTSPLTRAVL A VVRVRELL ...String:
MQSAARGCHA PAGLSSSLLR SRTYVPPCAP SSSGRVVGSR HITARAAGGK GGGKDDKAKE KADFSALWAL RIKNFFSSRR KYLQQAEQV GDDDKEKAFK AEIAKEERSI RELKDELVAI SMEEIEEQEK QGDPRPALAA TDIAQARMDL QTSPLTRAVL A VVRVRELL RALLLLPFSA VGGAVAAWQG LFNSQRYENF LMSEGERIWA WRNRSENERW FWEVFAWDRL IFPILVIVAW EY LVPNHLV WAVLAPLALL TWMSGRLPTP ATPEFWMLAY FGFYRKVWPD AAAWLQGYVV PLMGFA

UniProtKB: Uncharacterized protein

+
Macromolecule #12: FADL

MacromoleculeName: FADL / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 70.334234 KDa
SequenceString: MNTTRQMAAS TSCSSFTSLV APGTRLRTTV RAPAMQLRNG QRESGAAGTA ASASSSSCWR LAATALGQAQ LHSGARVSCM GGVIRSPAV LLRAGVPTRL PQSAHASPLA AVQPVTSTSG SVGELGAASR RTSSGAACSS SSSGSRSAVC TSGPVRGLGR P LPSAGLRR ...String:
MNTTRQMAAS TSCSSFTSLV APGTRLRTTV RAPAMQLRNG QRESGAAGTA ASASSSSCWR LAATALGQAQ LHSGARVSCM GGVIRSPAV LLRAGVPTRL PQSAHASPLA AVQPVTSTSG SVGELGAASR RTSSGAACSS SSSGSRSAVC TSGPVRGLGR P LPSAGLRR RRGASPIAAV GTPAVGSGSG VADSGANGST GSGNGADAAA AATAAAAPSN HHPPGSSNGD NGSSAAASAS TS SATAASA SDPAPEPEPA AASTSLDGLP ETQKYVYADE WGFSRVGADF PPGSHPSLFS QLLPQALFAF DARAAVAAVA VPL AAMAAG YGWLWYMHSI APVWQQALCA ALIGTGYAGL FKVAHECAMM RFIPQMPGLQ AALGTLLMAP ALYSLPSWRL HHLH HLLHT NMLWQDVWGW HPLTKVELAD EMVRSGGSGG AAMAAARLVL TTPIKLFASV GHWLRSWDGL DLRHFHPASY VEVLS GWAA PLAFAGLVLP AVVSAGGLSG FVSCYLAPWL VFHFWLSVLS LTAHTAPHIP WRAEGDGWDA GRAAVAGTVT LRLPRP LEV LLNNANYMLP QAVAPGLPMW SAPAAYAVLA ARLGPYLTEA SMSLKLLTNH VTRWQIYDEE AHTYRPMEEV VDEIEAD LQ QLAAAAQQAQ QQLAAQDQEA RAQQVEGREE GSGGGVPAVA

UniProtKB: Fatty acid desaturase domain-containing protein

+
Macromolecule #13: CrTam15

MacromoleculeName: CrTam15 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 15.265472 KDa
SequenceString:
MGGELAERKA SSASAGPRVV QMRREAIPAE LLLVKEDPSK LPAGVLQTRE QLKQAQRDIN WAGKREQVFA AVAAGWHLAS FALNLAFWG VEGMPPDRYW PTSPRIRLQI RPGRYGNMDG GQRVYMDYLA RSEGVPLN

UniProtKB: Uncharacterized protein

+
Macromolecule #14: CrTam49

MacromoleculeName: CrTam49 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 48.618184 KDa
SequenceString: MQSSIAHRSL AACPVQRNRF VPARCKLASN RIGPKLLSAA IAGTPEQPAT STSTPSSEAG TKDAAVVVGP ALVAKEVVST SGHSDAVPD PNFPELPLST NRAAGTQYLA IGAAYAVAAG AVAVAALQGP QLLLASPAAA DPWSSVLLGC VAATYLRAAG V FLQLKAAS ...String:
MQSSIAHRSL AACPVQRNRF VPARCKLASN RIGPKLLSAA IAGTPEQPAT STSTPSSEAG TKDAAVVVGP ALVAKEVVST SGHSDAVPD PNFPELPLST NRAAGTQYLA IGAAYAVAAG AVAVAALQGP QLLLASPAAA DPWSSVLLGC VAATYLRAAG V FLQLKAAS DAAELLCWRH QRLALTAAAY GMVAVLTQAA GLASPQLLGL QLLLSVASAA VVANVARSAW AVRPFVSSLT EG RSPGGVL AAVAGAMTGS VSTVAGLLLT TTIVVSLYGL FAAVFAPAPA LPVAVGAWPG TAAAAAVMDG SAAGLRRLAA GGL LLTAAA SHGLFDFAGS VKQQGPIDVA QAVKKMVVMD MAAPRIRTLK YFLPNPTIYS LLNLGFVAAA VLQSYFLYIA PAWG VNVNW DTALWGPMYG TAFLGLVYGL VALTKFDWSS VVDAVLRVAC WFAELTMWFW DTFVWKFSWS EKTRRA

UniProtKB: Uncharacterized protein

+
Macromolecule #15: Ctap7

MacromoleculeName: Ctap7 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 71.675305 KDa
SequenceString: MATTSAPTSE PWTFDLVGQL RQKFGLGPEN WDRFKGQAAE VPGADVPPSG AHVTLKDLSR PAESLPARAD EAAVQAALAD DGGWVGTPD PSKYAAGTTQ LSARELQEEV AKGNVMTWKD FKQQVSGLQG PEREALLALV AQRVAAERMF FTLEDGSKVS L WDLQQYVD ...String:
MATTSAPTSE PWTFDLVGQL RQKFGLGPEN WDRFKGQAAE VPGADVPPSG AHVTLKDLSR PAESLPARAD EAAVQAALAD DGGWVGTPD PSKYAAGTTQ LSARELQEEV AKGNVMTWKD FKQQVSGLQG PEREALLALV AQRVAAERMF FTLEDGSKVS L WDLQQYVD NNPELAALAA SVRRIAVADP EDPAGRPLPG GGASGLDRSR GLTGAAHMSG QEAEELELDW GQVGRGALWR RR PTRWLLG GLDGVKDWEL EAYAHEPLAN QLLGAKYGGR DPRAVVADPA YAADVLRAGP LLGMTFVLRA ARDLPLQEVA SSW RGLLGN YLQRQAPLSL PKAVRPAHLD PTDLNGVAWP ALLSRPAAAA HAAAEAEAAG AVPDDEMGVA WRVQSGKEAA ASVA AAQQL LQSLPDALCP GPSPAAWPLT GTKLVDEGGR NWRRGGSVWV TLQPEGGVLV QAQTGGVVGE QESYLLTHVQ GQEAL AGAV MSAFMGPQPL DPELAAAARS VLLVPANGFT AANKERDPNH PLYPSFTGVR PGRAPRDVAA YTLAGGRTPL LAAGGP GEA KLASELRTVM EAALAAAARA EAEALADAAT SPSSTSSRAA PAAALAEAEA AEARRARGRA AAAAVMAEGL RRLGPDA VA MLERTAAEAE APQGGGAVVV AGAGSASGEK GVGLTSSDIF SLARTLEQE

UniProtKB: Flagellar associated protein

+
Macromolecule #16: Tic22

MacromoleculeName: Tic22 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 40.685465 KDa
SequenceString: MDQAPGPLGH LQRSFEHVGR GLASQLEQVE QAFVPMREGV TRWLERTPLA QIIRNAQEGV ATQQRQRDQG PWAPMLASIA MSGAGPRPQ PVMDLAMAKD EVKARLAPVP VYTVANPKNE FVLVAGENNT QLGFFFFRKE DAEALIEKIR EENPRLARDS K ILRVPMDN ...String:
MDQAPGPLGH LQRSFEHVGR GLASQLEQVE QAFVPMREGV TRWLERTPLA QIIRNAQEGV ATQQRQRDQG PWAPMLASIA MSGAGPRPQ PVMDLAMAKD EVKARLAPVP VYTVANPKNE FVLVAGENNT QLGFFFFRKE DAEALIEKIR EENPRLARDS K ILRVPMDN VYEVFTTPRE QTGLQGIHFR FMPDMKQVAH ALQLYKDAGV PTRQFIGVPV FQAEGLTVTT RDMQYVPLFL CK EDLDIAV QSAYVQRNAA QIKLYKDKAD KYQADYDQIA SQLEAAANGR ERGGLESRLA KARVKLEAAR DKVESVERAP LPK VEVGSF EEVVMRMTAS AGNELAAWSQ VMFVAPELLR DSAGAGGKK

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Macromolecule #17: DnaJ

MacromoleculeName: DnaJ / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 50.842508 KDa
SequenceString: MGQFYSREFD GDPYVDLMRS LPERELVWWA QKVIWLAEGF TFVDHFARTY PRLLQHKCQR CKGAGVMTCP ACLGDARVSG GARRRAALA GLGGVAEGRS AHDHDHEAGA DGGCRVCGTA CAWDAE(SEP)EWM ERWGEWESRL AYYDKATGPL MDEWYED VL ...String:
MGQFYSREFD GDPYVDLMRS LPERELVWWA QKVIWLAEGF TFVDHFARTY PRLLQHKCQR CKGAGVMTCP ACLGDARVSG GARRRAALA GLGGVAEGRS AHDHDHEAGA DGGCRVCGTA CAWDAE(SEP)EWM ERWGEWESRL AYYDKATGPL MDEWYED VL NAGNLEED(TPO)P PVEDDPPGPE VTGRWAEHDR ALHKDKKRMA ALMRRWGHPY DADANLGYQI VDPTASMGEN VWNM AQVYN SLPPELNPLR TQHLADRGGG NTQAAVEAAR SAFDAQVVME AALLQNLEAA AQDLPKPHRL PPTAGTVACN ECGGA AWGY SFFPNTAVMF GLERPFWGDT LARLSKYWNP TQVADPARTG QLLPYGEGGL RRLLAAGGGG EDEEGGALEA VVGKAP ATT GRYRRDLELL LAHPELRDGA LRVPGGWGPE GGLQTYLRGQ QEEQARMQRR RDLAAEASPL ELAPAGK

UniProtKB: 4Fe-4S ferredoxin-type domain-containing protein

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Macromolecule #18: CrTam35

MacromoleculeName: CrTam35 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 34.403918 KDa
SequenceString: MVHVPFFGID LPEPRLAAVM PDAVYALVQG THKLGEYAHD LVFPPTPEDL RKLEQQVNAT IPREFDRVRQ RYAEGKIAND EQL(SEP)(SEP)ELED ASFNWYRRQL RTSVVGA(TPO)DE ELEDVAVRKL RLEPPALQAS LQERALAAAV TAAGGVDLA AEVADAAALA ...String:
MVHVPFFGID LPEPRLAAVM PDAVYALVQG THKLGEYAHD LVFPPTPEDL RKLEQQVNAT IPREFDRVRQ RYAEGKIAND EQL(SEP)(SEP)ELED ASFNWYRRQL RTSVVGA(TPO)DE ELEDVAVRKL RLEPPALQAS LQERALAAAV TAAGGVDLA AEVADAAALA ALAEQEAETR RLLAARQARL ADLRKQLRPA HRQAIGTITN ASAGMLVGVL VVKSVLTLVR RLFRKKRPAG AKAPAGAAA RRQGSVQSSA AQLQSLAAPA ARQQQQSAAA ALASAQQAAM RPQAGASASA AAAASGKAAA SKEQPKAGAA A AAADAGAK KTRIVKKR

UniProtKB: Uncharacterized protein

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Macromolecule #19: CrTam31

MacromoleculeName: CrTam31 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 31.245342 KDa
SequenceString: MASTARRKPV PEAMSED(SEP)VE QEFGGDY(SEP)EA SLEYIRALGP KKGAPFAEVD PAEQAAALPP SQAECEAAKA VL ENYKRDI DALKTYRPSE QERAAIDARR ALALEATPEE LTWLKMREYY ATQRAAAADG AASTSAPGAG DAASAAADND VAA LRAEML ...String:
MASTARRKPV PEAMSED(SEP)VE QEFGGDY(SEP)EA SLEYIRALGP KKGAPFAEVD PAEQAAALPP SQAECEAAKA VL ENYKRDI DALKTYRPSE QERAAIDARR ALALEATPEE LTWLKMREYY ATQRAAAADG AASTSAPGAG DAASAAADND VAA LRAEML RVAMEALGSA TETEELEARA RSHLAASSAG RQWSRGVAGG AGAAASGEAS AAVLAAAADA AVASVMVGTG ARYR DDYLE EERLTRQVAK QRGQRSQAFA LSLAAAVGVS VARWWLRRGR GGAGAGGGSG GPSRSRSTQQ MQ

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Macromolecule #20: UNK

MacromoleculeName: UNK / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 7.337035 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

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Macromolecule #21: UNK

MacromoleculeName: UNK / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 13.631775 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) Y(UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) Y(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)VHVFRK (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #22: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 22 / Number of copies: 5 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

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Macromolecule #23: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / type: ligand / ID: 23 / Number of copies: 4 / Formula: ANP
Molecular weightTheoretical: 506.196 Da
Chemical component information

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

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Macromolecule #24: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 24 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #25: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

MacromoleculeName: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
type: ligand / ID: 25 / Number of copies: 3 / Formula: SQD
Molecular weightTheoretical: 795.116 Da
Chemical component information

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

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Macromolecule #26: DIACYL GLYCEROL

MacromoleculeName: DIACYL GLYCEROL / type: ligand / ID: 26 / Number of copies: 2 / Formula: DGA
Molecular weightTheoretical: 625.018 Da
Chemical component information

ChemComp-DGA:
DIACYL GLYCEROL

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Macromolecule #27: CHOLESTEROL HEMISUCCINATE

MacromoleculeName: CHOLESTEROL HEMISUCCINATE / type: ligand / ID: 27 / Number of copies: 2 / Formula: Y01
Molecular weightTheoretical: 486.726 Da
Chemical component information

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

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Macromolecule #28: Beta-Sitosterol

MacromoleculeName: Beta-Sitosterol / type: ligand / ID: 28 / Number of copies: 2 / Formula: A1LXL
Molecular weightTheoretical: 416.723 Da

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Macromolecule #29: DIGALACTOSYL DIACYL GLYCEROL (DGDG)

MacromoleculeName: DIGALACTOSYL DIACYL GLYCEROL (DGDG) / type: ligand / ID: 29 / Number of copies: 2 / Formula: DGD
Molecular weightTheoretical: 949.299 Da
Chemical component information

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

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Macromolecule #30: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 30 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.4000000000000001 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 172550
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelChain - Source name: Other / Chain - Initial model type: in silico model
Output model

PDB-8xqw:
Cryo-EM structure of the Ycf2-FtsHi motor complex from Chlamydomonas reinhardtii in AMPPNP bound state

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