EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Substrate translocation and inhibition in human dicarboxylate transporter NaDC3.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 32, Issue 3, Page 502-512, Year 2025
掲載日	2024年12月2日
著者	Yan Li / Jinmei Song / Vedrana Mikusevic / Jennifer J Marden / Alissa Becerril / Huihui Kuang / Bing Wang / William J Rice / Joseph A Mindell / Da-Neng Wang /
PubMed 要旨	The human high-affinity sodium-dicarboxylate cotransporter (NaDC3) imports various substrates into the cell as tricarboxylate acid cycle intermediates, lipid biosynthesis precursors and signaling ...The human high-affinity sodium-dicarboxylate cotransporter (NaDC3) imports various substrates into the cell as tricarboxylate acid cycle intermediates, lipid biosynthesis precursors and signaling molecules. Understanding the cellular signaling process and developing inhibitors require knowledge of the structural basis of the dicarboxylate specificity and inhibition mechanism of NaDC3. To this end, we determined the cryo-electron microscopy structures of NaDC3 in various dimers, revealing the protomer in three conformations: outward-open C, outward-occluded C and inward-open C. A dicarboxylate is first bound and recognized in C and how the substrate interacts with NaDC3 in C likely helps to further determine the substrate specificity. A phenylalanine from the scaffold domain interacts with the bound dicarboxylate in the C state and modulates the kinetic barrier to the transport domain movement. Structural comparison of an inhibitor-bound structure of NaDC3 to that of the sodium-dependent citrate transporter suggests ways for making an inhibitor that is specific for NaDC3.
リンク	Nat Struct Mol Biol / PubMed:39622972 / PubMed Central
手法	EM (単粒子)
解像度	2.09 - 2.92 Å
構造データ	42614 8uvb EMDB-42614, PDB-8uvb: Structure of NaCT-PF4a complex 手法: EM (単粒子) / 解像度: 2.13 Å 42615 8uvc EMDB-42615, PDB-8uvc: Structure of NaDC3-aKG complex 手法: EM (単粒子) / 解像度: 2.09 Å 42616 8uvd EMDB-42616, PDB-8uvd: Structure of NaDC3-PF4a complex 手法: EM (単粒子) / 解像度: 2.16 Å 42617 8uve EMDB-42617, PDB-8uve: Structure of NaDC3-Succinate complex in Coo-Ci conformation 手法: EM (単粒子) / 解像度: 2.6 Å 42618 8uvf EMDB-42618, PDB-8uvf: Structure of NaDC3-DMS complex in Ci-Ci conformation 手法: EM (単粒子) / 解像度: 2.17 Å 42619 8uvg EMDB-42619, PDB-8uvg: Structure of NaDC3-DMS complex in Co-Ci conformation 手法: EM (単粒子) / 解像度: 2.92 Å 42620 8uvh EMDB-42620, PDB-8uvh: Structure of NaCT-succ complex 手法: EM (単粒子) / 解像度: 2.33 Å 42621 8uvi EMDB-42621, PDB-8uvi: Structure of NaDC3-Succinate complex in Coo-Coo conformation 手法: EM (単粒子) / 解像度: 2.53 Å
化合物	ChemComp-NA: Unknown entry / ナトリウムカチオン化合物画像なし ChemComp-XKC: Unknown entry ChemComp-AKG: 2-OXOGLUTARIC ACID / α-ケトグルタル酸 ChemComp-C14: TETRADECANE / テトラデカン ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM ChemComp-CLR: CHOLESTEROL / コレステロ-ル ChemComp-SIN: SUCCINIC ACID / コハク酸化合物画像なし ChemComp-XKH*: Unknown entry
由来	homo sapiens (ヒト)
キーワード	TRANSPORT PROTEIN / Na(+)/citrate cotransporter(NaCT) / Solute carries / Elevator type alternating access / membrane protein / Na(+)/dicarboxylate cotransporter(NaDC3)