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Structure paper

TitleCryo-EM of human KIF1A reveals its mechanism of motility and the effect of its pathogenic variant P305L
Journal, issue, pagesTo Be Published
Publish dateOct 31, 2023 (structure data deposition date)
AuthorsBenoit MPMH / Rao L / Asenjo AB / Gennerich A / Sosa H
External linksSearch PubMed
MethodsEM (helical sym.)
Resolution2.7 - 3.4 Å
Structure data

EMDB-42543, PDB-8utn:
KIF1A[1-393] AMP-PNP bound two-heads-bound state in complex with a microtubule (class T23L1)
Method: EM (helical sym.) / Resolution: 3.1 Å

EMDB-42544, PDB-8uto:
KIF1A[1-393] AMP-PNP bound two-heads-bound state in complex with a microtubule - class T2L1
Method: EM (helical sym.) / Resolution: 3.2 Å

EMDB-42545, PDB-8utp:
KIF1A[1-393] - AMP-PNP two-heads-bound state in complex with a microtubule - class T3L1
Method: EM (helical sym.) / Resolution: 3.2 Å

EMDB-42546, PDB-8utq:
KIF1A[1-393] AMP-PNP bound one-head-bound state in complex with a microtubule - class T1L02*
Method: EM (helical sym.) / Resolution: 3.1 Å

EMDB-42547, PDB-8utr:
KIF1A[1-393] ADP bound in complex with a microtubule
Method: EM (helical sym.) / Resolution: 3.3 Å

EMDB-42548, PDB-8uts:
KIF1A[1-393] APO in complex with a microtubule
Method: EM (helical sym.) / Resolution: 2.7 Å

EMDB-42549, PDB-8utt:
KIF1A[1-393] P305L mutant AMP-PNP bound two-heads-bound state in complex with a microtubule
Method: EM (helical sym.) / Resolution: 3.1 Å

EMDB-42550: KIF1A[1-393] AMP-PNP bound one-head-bound state in complex with a microtubule - class T1L02*
PDB-8utu: KIF1A[1-393] P305L mutant AMP-PNP bound one and two heads bound states merged, in complex with a microtubule
Method: EM (helical sym.) / Resolution: 3.0 Å

EMDB-42551, PDB-8utv:
KIF1A[1-393] P305L mutant ADP bound in complex with a microtubule
Method: EM (helical sym.) / Resolution: 3.0 Å

EMDB-42552, PDB-8utw:
KIF1A[1-393] P305L mutant APO in complex with a microtubule
Method: EM (helical sym.) / Resolution: 3.4 Å

EMDB-42553, PDB-8uty:
KIF1A[1-393] P364L mutant AMP-PNP bound two-heads-bound state in complex with a microtubule
Method: EM (helical sym.) / Resolution: 3.3 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • homo sapiens (human)
  • sus scrofa (pig)
KeywordsMOTOR PROTEIN / KIF1A / kinesin / motility / microtubule / tubulin

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