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TitleChromosome end protection by RAP1-mediated inhibition of DNA-PK.
Journal, issue, pagesNature, Vol. 642, Issue 8069, Page 1090-1096, Year 2025
Publish dateApr 16, 2025
AuthorsPatrik Eickhoff / Ceylan Sonmez / Charlotte E L Fisher / Oviya Inian / Theodoros I Roumeliotis / Angela Dello Stritto / Jörg Mansfeld / Jyoti S Choudhary / Sebastian Guettler / Francisca Lottersberger / Max E Douglas /
PubMed AbstractDuring classical non-homologous end joining (cNHEJ), DNA-dependent protein kinase (DNA-PK) encapsulates free DNA ends, forming a recruitment platform for downstream end-joining factors including ...During classical non-homologous end joining (cNHEJ), DNA-dependent protein kinase (DNA-PK) encapsulates free DNA ends, forming a recruitment platform for downstream end-joining factors including ligase 4 (LIG4). DNA-PK can also bind telomeres and regulate their resection, but does not initiate cNHEJ at this position. How the end-joining process is regulated in this context-specific manner is currently unclear. Here we show that the shelterin components TRF2 and RAP1 form a complex with DNA-PK that directly represses its end-joining function at telomeres. Biochemical experiments and cryo-electron microscopy reveal that when bound to TRF2, RAP1 establishes a network of interactions with KU and DNA that prevents DNA-PK from recruiting LIG4. In mouse and human cells, RAP1 is redundant with the Apollo nuclease in repressing cNHEJ at chromosome ends, demonstrating that the inhibition of DNA-PK prevents telomere fusions in parallel with overhang-dependent mechanisms. Our experiments show that the end-joining function of DNA-PK is directly and specifically repressed at telomeres, establishing a molecular mechanism for how individual linear chromosomes are maintained in mammalian cells.
External linksNature / PubMed:40240611 / PubMed Central
MethodsEM (single particle)
Resolution3.32 - 3.58 Å
Structure data

19065

8rd4

EMDB-19065, PDB-8rd4:
Telomeric RAP1:DNA-PK complex
Method: EM (single particle) / Resolution: 3.58 Å

EMDB-19245: Telomeric RAP1:DNA-PK complex consensus map
Method: EM (single particle) / Resolution: 3.58 Å

EMDB-19249: RAP1:KU local refinement of telomeric RAP1:DNA-PK complex
Method: EM (single particle) / Resolution: 3.32 Å

EMDB-19252: DNA-PKcs local refinement of telomeric RAP1:DNA-PK complex
Method: EM (single particle) / Resolution: 3.4 Å

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN / Telomere NHEJ BRCT domain SAP domain

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