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TitleHow sensor Amt-like proteins integrate ammonium signals.
Journal, issue, pagesSci Adv, Vol. 10, Issue 23, Page eadm9441, Year 2024
Publish dateJun 7, 2024
AuthorsTobias Pflüger / Mathias Gschell / Lin Zhang / Volodymyr Shnitsar / Annas J Zabadné / Paul Zierep / Stefan Günther / Oliver Einsle / Susana L A Andrade /
PubMed AbstractUnlike aquaporins or potassium channels, ammonium transporters (Amts) uniquely discriminate ammonium from potassium and water. This feature has certainly contributed to their repurposing as ammonium ...Unlike aquaporins or potassium channels, ammonium transporters (Amts) uniquely discriminate ammonium from potassium and water. This feature has certainly contributed to their repurposing as ammonium receptors during evolution. Here, we describe the ammonium receptor Sd-Amt1, where an Amt module connects to a cytoplasmic diguanylate cyclase transducer module via an HAMP domain. Structures of the protein with and without bound ammonium were determined to 1.7- and 1.9-Ångstrom resolution, depicting the ON and OFF states of the receptor and confirming the presence of a binding site for two ammonium cations that is pivotal for signal perception and receptor activation. The transducer domain was disordered in the crystals, and an AlphaFold2 prediction suggests that the helices linking both domains are flexible. While the sensor domain retains the trimeric fold formed by all Amt family members, the HAMP domains interact as pairs and serve to dimerize the transducer domain upon activation.
External linksSci Adv / PubMed:38838143 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.7 - 2.53 Å
Structure data

18549

8qpf

EMDB-18549, PDB-8qpf:
Ammonium Transporter Amt1 from Shewanella denitrificans
Method: EM (single particle) / Resolution: 2.53 Å

PDB-8qj3:
Receptor Sd-Amt1 (OFF-state)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-8qj4:
Receptor Sd-Amt1 (ON-state)
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-HOH:
WATER

ChemComp-NH4:
AMMONIUM ION

Source
  • shewanella denitrificans (bacteria)
  • shewanella denitrificans os217 (bacteria)
KeywordsSIGNALING PROTEIN / Ammonium receptor / OFF-state / Shewanella denitrificans / Sd-Amt1 / diguanylate cyclase / Amt / ON-state / TRANSPORT PROTEIN / Ammonium Transporter Amt1

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