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Structure paper

TitleCrystal structures of OPR3 wildtype and variants with NAD(P)H
Journal, issue, pagesTo Be Published
Publish dateSep 27, 2023 (structure data deposition date)
AuthorsKerschbaumer, B. / Bijelic, A. / Macheroux, P.
External linksSearch PubMed
MethodsX-ray diffraction
Resolution1.91 - 2 Å
Structure data

PDB-8qnk:
OPR3 variant R283D in complex with NADPH4
Method: X-RAY DIFFRACTION / Resolution: 1.91 Å

PDB-8qnp:
OPR3 with redesigned loop 6 (9aa) in complex with NADH4
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-5J8:
[[(2R,3S,4R,5R)-5-(5-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methyl hydrogen phosphate

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-WI6:
1,4,5,6-Tetrahydronicotinamide adenine dinucleotide

ChemComp-SO4:
SULFATE ION

Source
  • solanum lycopersicum (tomato)
KeywordsFLAVOPROTEIN / Old yellow enzyme / flavoenzyme / ene-reductase / NADPH / Oly yellow enzyme

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