EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The cohesin ATPase cycle is mediated by specific conformational dynamics and interface plasticity of SMC1A and SMC3 ATPase domains.
ジャーナル・号・ページ	Cell Rep, Vol. 43, Issue 9, Page 114656, Year 2024
掲載日	2024年9月24日
著者	Marina Vitoria Gomes / Pauline Landwerlin / Marie-Laure Diebold-Durand / Tajith B Shaik / Alexandre Durand / Edouard Troesch / Chantal Weber / Karl Brillet / Marianne Victoria Lemée / Christophe Decroos / Ludivine Dulac / Pierre Antony / Erwan Watrin / Eric Ennifar / Christelle Golzio / Christophe Romier /
PubMed 要旨	Cohesin is key to eukaryotic genome organization and acts throughout the cell cycle in an ATP-dependent manner. The mechanisms underlying cohesin ATPase activity are poorly understood. Here, we ...Cohesin is key to eukaryotic genome organization and acts throughout the cell cycle in an ATP-dependent manner. The mechanisms underlying cohesin ATPase activity are poorly understood. Here, we characterize distinct steps of the human cohesin ATPase cycle and show that the SMC1A and SMC3 ATPase domains undergo specific but concerted structural rearrangements along this cycle. Specifically, whereas the proximal coiled coil of the SMC1A ATPase domain remains conformationally stable, that of the SMC3 displays an intrinsic flexibility. The ATP-dependent formation of the heterodimeric SMC1A/SMC3 ATPase module (engaged state) favors this flexibility, which is counteracted by NIPBL and DNA binding (clamped state). Opening of the SMC3/RAD21 interface (open-engaged state) stiffens the SMC3 proximal coiled coil, thus constricting together with that of SMC1A the ATPase module DNA-binding chamber. The plasticity of the ATP-dependent interface between the SMC1A and SMC3 ATPase domains enables these structural rearrangements while keeping the ATP gate shut. VIDEO ABSTRACT.
リンク	Cell Rep / PubMed:39240714
手法	EM (単粒子) / X線回折
解像度	1.36 - 4.4 Å
構造データ	17331 8p0a EMDB-17331, PDB-8p0a: Human Cohesin ATPase module 手法: EM (単粒子) / 解像度: 3.67 Å 17820 8pq5 EMDB-17820, PDB-8pq5: Human Cohesin ATPase module with an open DNA exit gate 手法: EM (単粒子) / 解像度: 4.4 Å PDB-8ro6: Human cohesin SMC1A-HD(longCC)/RAD21-C complex - Apo closed P-loop conformation 手法: X-RAY DIFFRACTION / 解像度: 2.2 Å PDB-8ro7: Human cohesin SMC1A-HD(longCC)/RAD21-C complex - Open/closed P-loop conformation 手法: X-RAY DIFFRACTION / 解像度: 2.0929 Å PDB-8ro8: Human cohesin SMC1A-HD(longCC-EQ)/RAD21-C complex - Apo closed P-loop conformation 手法: X-RAY DIFFRACTION / 解像度: 1.902 Å PDB-8ro9: Human cohesin SMC1A-HD(longCC-EQ)/RAD21-C complex - Open/closed P-loop conformation 手法: X-RAY DIFFRACTION / 解像度: 1.767 Å PDB-8roa: Human cohesin SMC1A-HD(longCC-EQ)/RAD21-C complex - ADP-bound conformation 手法: X-RAY DIFFRACTION / 解像度: 2.4355 Å PDB-8rob: Human cohesin SMC1A-HD(longCC-EQ)/RAD21-C complex - ATPgS-Mg-bound conformation 手法: X-RAY DIFFRACTION / 解像度: 2.5019 Å PDB-8roc: Human cohesin SMC1A-HD(shortCC-EQ)/RAD21-C complex - Apo closed P-loop conformation 手法: X-RAY DIFFRACTION / 解像度: 1.85 Å PDB-8rod: Human cohesin SMC1A-HD(shortCC-EQ)/RAD21-C complex - Open/closed P-loop conformation 手法: X-RAY DIFFRACTION / 解像度: 1.5 Å PDB-8roe: Human cohesin SMC1A-HD(shortCC-EQ)/RAD21-C complex - ADP-bound conformation 手法: X-RAY DIFFRACTION / 解像度: 1.36 Å PDB-8rof: Human cohesin SMC1A-HD(shortCC-EQ)/RAD21-C complex - ADP-Mg-bound conformation 手法: X-RAY DIFFRACTION / 解像度: 1.65 Å PDB-8rog: Human cohesin SMC1A-HD(shortCC-EQ)/RAD21-C complex - ATPgS-Mg-bound conformation 手法: X-RAY DIFFRACTION / 解像度: 1.94 Å PDB-8roh: Human cohesin SMC3-HD(EQ)/RAD21-N complex - Apo conformation 手法: X-RAY DIFFRACTION / 解像度: 2.6 Å PDB-8roi: Human cohesin SMC3-HD(EQ)/RAD21-N complex - ADP-bound conformation 手法: X-RAY DIFFRACTION / 解像度: 2.45 Å PDB-8roj: Human cohesin SMC3-HD/RAD21-N complex - ADP-Mg-bound conformation 手法: X-RAY DIFFRACTION / 解像度: 3 Å PDB-8rok: Human cohesin SMC3-HD(EQ)/RAD21-N complex - ATP-Mg-bound conformation - Form 1 手法: X-RAY DIFFRACTION / 解像度: 2.25 Å PDB-8rol: Human cohesin SMC3-HD(EQ)/RAD21-N complex - ATP-Mg-bound conformation - Form 2 手法: X-RAY DIFFRACTION / 解像度: 3.11 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry ChemComp-HOH: WATER ChemComp-B3P: 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH緩衝剤YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, エネルギー貯蔵分子YM ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-SO4: SULFATE ION ChemComp-GOL: GLYCEROL
由来	homo sapiens (ヒト)
キーワード	DNA BINDING PROTEIN / 3D genome organization / Chromatin / Cohesin / ATPase activity / ATPase cycle / Cell cycle / DNA binding / NUCLEAR PROTEIN