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TitleStructural insights into the functional mechanism of the ubiquitin ligase E6AP.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 3531, Year 2024
Publish dateApr 26, 2024
AuthorsZhen Wang / Fengying Fan / Zhihai Li / Fei Ye / Qingxia Wang / Rongchao Gao / Jiaxuan Qiu / Yixin Lv / Min Lin / Wenwen Xu / Cheng Luo / Xuekui Yu /
PubMed AbstractE6AP dysfunction is associated with Angelman syndrome and Autism spectrum disorder. Additionally, the host E6AP is hijacked by the high-risk HPV E6 to aberrantly ubiquitinate the tumor suppressor ...E6AP dysfunction is associated with Angelman syndrome and Autism spectrum disorder. Additionally, the host E6AP is hijacked by the high-risk HPV E6 to aberrantly ubiquitinate the tumor suppressor p53, which is linked with development of multiple types of cancer, including most cervical cancers. Here we show that E6AP and the E6AP/E6 complex exist, respectively, as a monomer and a dimer of the E6AP/E6 protomer. The short α1-helix of E6AP transforms into a longer helical structure when in complex with E6. The extended α1-helices of the dimer intersect symmetrically and contribute to the dimerization. The two protomers sway around the crossed region of the two α1-helices to promote the attachment and detachment of substrates to the catalytic C-lobe of E6AP, thus facilitating ubiquitin transfer. These findings, complemented by mutagenesis analysis, suggest that the α1-helix, through conformational transformations, controls the transition between the inactive monomer and the active dimer of E6AP.
External linksNat Commun / PubMed:38670961 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 5.1 Å
Structure data

36599

8jrn

EMDB-36599, PDB-8jrn:
Structure of E6AP-E6 complex in Att1 state
Method: EM (single particle) / Resolution: 2.6 Å

36600

8jro

EMDB-36600, PDB-8jro:
Structure of E6AP-E6 complex in Att2 state
Method: EM (single particle) / Resolution: 3.01 Å

36601

8jrp

EMDB-36601, PDB-8jrp:
Structure of E6AP-E6 complex in Att3 state
Method: EM (single particle) / Resolution: 3.58 Å

36602

8jrq

EMDB-36602, PDB-8jrq:
Structure of E6AP-E6 complex in Det1 state
Method: EM (single particle) / Resolution: 4.15 Å

36603

8jrr

EMDB-36603, PDB-8jrr:
Structure of E6AP-E6 complex in Det2 state
Method: EM (single particle) / Resolution: 4.35 Å

EMDB-36604: Structure of human full-length E6AP
Method: EM (single particle) / Resolution: 5.1 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • human papillomavirus 16
KeywordsLIGASE/ONCOPROTEIN / Complex / Viral protein / Tumor / LIGASE-ONCOPROTEIN complex

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