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Structure paper

TitleStructures of Tetrahymena thermophila respiratory megacomplexes on the tubular mitochondrial cristae.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 2542, Year 2023
Publish dateMay 29, 2023
AuthorsFangzhu Han / Yiqi Hu / Mengchen Wu / Zhaoxiang He / Hongtao Tian / Long Zhou /
PubMed AbstractTetrahymena thermophila, a classic ciliate model organism, has been shown to possess tubular mitochondrial cristae and highly divergent electron transport chain involving four transmembrane protein ...Tetrahymena thermophila, a classic ciliate model organism, has been shown to possess tubular mitochondrial cristae and highly divergent electron transport chain involving four transmembrane protein complexes (I-IV). Here we report cryo-EM structures of its ~8 MDa megacomplex IV+ (I + III+ II), as well as a ~ 10.6 MDa megacomplex (IV + I + III+ II) at lower resolution. In megacomplex IV+ (I + III+ II), each CIV protomer associates one copy of supercomplex I + III and one copy of CII, forming a half ring-shaped architecture that adapts to the membrane curvature of mitochondrial cristae. Megacomplex (IV+ I + III+ II) defines the relative position between neighbouring half rings and maintains the proximity between CIV and CIII cytochrome c binding sites. Our findings expand the current understanding of divergence in eukaryotic electron transport chain organization and how it is related to mitochondrial morphology.
External linksNat Commun / PubMed:37248254 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 6.77 Å
Structure data

34373

8gym

EMDB-34373, PDB-8gym:
Cryo-EM structure of Tetrahymena thermophila respiratory mega-complex MC IV2+(I+III2+II)2
Method: EM (single particle) / Resolution: 2.96 Å

EMDB-34380: Cryo-EM map of Tetrahymena thermophila respiratory complex I peripheral arm
Method: EM (single particle) / Resolution: 2.96 Å

EMDB-34381: Cryo-EM map of Tetrahymena thermophila respiratory complex I membrane arm distal portion
Method: EM (single particle) / Resolution: 2.83 Å

EMDB-34382: Cryo-EM map of Tetrahymena thermophila respiratory complex I membrane arm proximal portion
Method: EM (single particle) / Resolution: 2.8 Å

EMDB-34383: Cryo-EM map of Tetrahymena thermophila respiratory complex IV
Method: EM (single particle) / Resolution: 2.89 Å

EMDB-34384: Cryo-EM map of Tetrahymena thermophila respiratory complex III
Method: EM (single particle) / Resolution: 2.86 Å

EMDB-34385: Cryo-EM map of Tetrahymena thermophila respiratory complex II
Method: EM (single particle) / Resolution: 3.22 Å

EMDB-34386: Cryo-EM map of Tetrahymena thermophila respiratory complex I membrane arm distal portion, digitonin sample
Method: EM (single particle) / Resolution: 2.92 Å

34403

8gzu

EMDB-34403, PDB-8gzu:
Cryo-EM structure of Tetrahymena thermophila respiratory Megacomplex MC (IV2+I+III2+II)2
Method: EM (single particle) / Resolution: 4.18 Å

EMDB-34404: Cryo-EM structure of Tetrahymena thermophila respiratory super-complex SC IV2+I+III2+II
Method: EM (single particle) / Resolution: 4.18 Å

EMDB-34405: Cryo-EM structure of Tetrahymena thermophila respiratory super-complex SC IV2+I+III2+II, copy2
Method: EM (single particle) / Resolution: 6.77 Å

EMDB-36375: Cryo-EM map of Tetrahymena thermophila megacomplex IV2+(I+III2+II)2
Method: EM (single particle) / Resolution: 2.96 Å

Chemicals

ChemComp-HEC:
HEME C / Heme C

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM / Phosphatidylcholine

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

ChemComp-HEA:
HEME-A / Heme A

ChemComp-CU:
COPPER (II) ION / Copper

ChemComp-MG:
Unknown entry

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM / Phosphatidylethanolamine

ChemComp-P5S:
O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine / Phosphatidylserine

ChemComp-PX2:
1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM / Flavin adenine dinucleotide

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-F3S:
FE3-S4 CLUSTER / Iron–sulfur cluster

ChemComp-U10:
UBIQUINONE-10 / Coenzyme Q10

ChemComp-ZN:
Unknown entry

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

ChemComp-T7X:
Phosphatidylinositol / Phosphatidylinositol

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

Source
  • tetrahymena thermophila sb210 (eukaryote)
  • Tetrahymena thermophila (eukaryote)
KeywordsELECTRON TRANSPORT / Electron transport chain / supercomplex / membrane protein / Tetrahymena thermophila

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