EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A tradeoff between enterovirus A71 particle stability and cell entry.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 7450, Year 2023
掲載日	2023年11月17日
著者	Adam Catching / Ming Te Yeh / Simone Bianco / Sara Capponi / Raul Andino /
PubMed 要旨	A central role of viral capsids is to protect the viral genome from the harsh extracellular environment while facilitating initiation of infection when the virus encounters a target cell. Viruses are ...A central role of viral capsids is to protect the viral genome from the harsh extracellular environment while facilitating initiation of infection when the virus encounters a target cell. Viruses are thought to have evolved an optimal equilibrium between particle stability and efficiency of cell entry. In this study, we genetically perturb this equilibrium in a non-enveloped virus, enterovirus A71 to determine its structural basis. We isolate a single-point mutation variant with increased particle thermotolerance and decreased efficiency of cell entry. Using cryo-electron microscopy and molecular dynamics simulations, we determine that the thermostable native particles have acquired an expanded conformation that results in a significant increase in protein dynamics. Examining the intermediate states of the thermostable variant reveals a potential pathway for uncoating. We propose a sequential release of the lipid pocket factor, followed by internal VP4 and ultimately the viral RNA.
リンク	Nat Commun / PubMed:37978288 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 14.0 Å
構造データ	27850 8e2x EMDB-27850, PDB-8e2x: Purification of Enterovirus A71, strain 4643, WT capsid 手法: EM (単粒子) / 解像度: 3.3 Å 27851 8e2y EMDB-27851, PDB-8e2y: Purification of Enterovirus A71, strain 4643, WT capsid 手法: EM (単粒子) / 解像度: 8.0 Å 27853 8e31 EMDB-27853, PDB-8e31: Purification of Enterovirus A71, strain 4643, WT capsid 手法: EM (単粒子) / 解像度: 14.0 Å 27859 8e38 EMDB-27859, PDB-8e38: Purification of Enterovirus A71, strain 4643, WT capsid 手法: EM (単粒子) / 解像度: 4.2 Å 27860 8e39 EMDB-27860, PDB-8e39: Purification of Enterovirus A71, strain 4643, WT capsid 手法: EM (単粒子) / 解像度: 3.1 Å 27861 8e3a EMDB-27861, PDB-8e3a: Purification of Enterovirus A71, strain 4643, WT capsid 手法: EM (単粒子) / 解像度: 7.4 Å 27862 8e3b EMDB-27862, PDB-8e3b: Purification of Enterovirus A71, strain 4643, WT capsid 手法: EM (単粒子) / 解像度: 5.9 Å 27863 8e3c EMDB-27863, PDB-8e3c: Purification of Enterovirus A71, strain 4643, WT capsid 手法: EM (単粒子) / 解像度: 7.1 Å
化合物	ChemComp-SPH: SPHINGOSINE
由来	human enterovirus 71 (エンテロウイルス) enterovirus a71 (エンテロウイルス)
キーワード	VIRUS / enterovirus / thermostability / capsid