Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Lipid flipping in the omega-3 fatty-acid transporter.
Journal, issue, pages	Nat Commun, Vol. 14, Issue 1, Page 2571, Year 2023
Publish date	May 8, 2023
Authors	Chi Nguyen / Hsiang-Ting Lei / Louis Tung Faat Lai / Marc J Gallenito / Xuelang Mu / Doreen Matthies / Tamir Gonen /
PubMed Abstract	Mfsd2a is the transporter for docosahexaenoic acid (DHA), an omega-3 fatty acid, across the blood brain barrier (BBB). Defects in Mfsd2a are linked to ailments from behavioral and motor dysfunctions ...Mfsd2a is the transporter for docosahexaenoic acid (DHA), an omega-3 fatty acid, across the blood brain barrier (BBB). Defects in Mfsd2a are linked to ailments from behavioral and motor dysfunctions to microcephaly. Mfsd2a transports long-chain unsaturated fatty-acids, including DHA and α-linolenic acid (ALA), that are attached to the zwitterionic lysophosphatidylcholine (LPC) headgroup. Even with the recently determined structures of Mfsd2a, the molecular details of how this transporter performs the energetically unfavorable task of translocating and flipping lysolipids across the lipid bilayer remains unclear. Here, we report five single-particle cryo-EM structures of Danio rerio Mfsd2a (drMfsd2a): in the inward-open conformation in the ligand-free state and displaying lipid-like densities modeled as ALA-LPC at four distinct positions. These Mfsd2a snapshots detail the flipping mechanism for lipid-LPC from outer to inner membrane leaflet and release for membrane integration on the cytoplasmic side. These results also map Mfsd2a mutants that disrupt lipid-LPC transport and are associated with disease.
External links	Nat Commun / PubMed:37156797 / PubMed Central
Methods	EM (single particle)
Resolution	2.9 - 4.1 Å
Structure data	27148 8d2s EMDB-27148, PDB-8d2s: Zebrafish MFSD2A isoform B in inward open ligand bound conformation Method: EM (single particle) / Resolution: 2.9 Å 27149 8d2t EMDB-27149, PDB-8d2t: Zebrafish MFSD2A isoform B in inward open ligand-free conformation Method: EM (single particle) / Resolution: 3.4 Å 27150 8d2u EMDB-27150, PDB-8d2u: Zebrafish MFSD2A isoform B in inward open ligand 1A conformation Method: EM (single particle) / Resolution: 3.3 Å 27151 8d2v EMDB-27151, PDB-8d2v: Zebrafish MFSD2A isoform B in inward open ligand 1B conformation Method: EM (single particle) / Resolution: 4.1 Å 27152 8d2w EMDB-27152, PDB-8d2w: Zebrafish MFSD2A isoform B in inward open ligand 2B conformation Method: EM (single particle) / Resolution: 3.4 Å 27153 8d2x EMDB-27153, PDB-8d2x: Zebrafish MFSD2A isoform B in inward open ligand 3C conformation Method: EM (single particle) / Resolution: 3.4 Å
Chemicals	ChemComp-ZGS: [(2~{R})-2-oxidanyl-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] (9~{Z},12~{Z},15~{Z})-octadeca-9,12,15-trienoate ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / detergentYM ChemComp-NA: Unknown entry ChemComp-HOH*: WATER
Source	danio rerio (zebrafish) mus musculus (house mouse)
Keywords	LIPID TRANSPORT / omega-3 fatty acid / membrane protein / Fab