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-Structure paper
Title | Principles of mitoribosomal small subunit assembly in eukaryotes. |
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Journal, issue, pages | Nature, Vol. 614, Issue 7946, Page 175-181, Year 2023 |
Publish date | Dec 8, 2022 |
Authors | Nathan J Harper / Chloe Burnside / Sebastian Klinge / |
PubMed Abstract | Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is ...Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is essential for ATP production and cellular metabolism. Here we used cryo-electron microscopy to determine nine structures of native yeast and human mitoribosomal small subunit assembly intermediates, illuminating the mechanistic basis for how GTPases are used to control early steps of decoding centre formation, how initial rRNA folding and processing events are mediated, and how mitoribosomal proteins have active roles during assembly. Furthermore, this series of intermediates from two species with divergent mitoribosomal architecture uncovers both conserved principles and species-specific adaptations that govern the maturation of mitoribosomal small subunits in eukaryotes. By revealing the dynamic interplay between assembly factors, mitoribosomal proteins and rRNA that are required to generate functional subunits, our structural analysis provides a vignette for how molecular complexity and diversity can evolve in large ribonucleoprotein assemblies. |
External links | Nature / PubMed:36482135 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.36 - 3.8 Å |
Structure data | EMDB-26966, PDB-8csp: EMDB-26967, PDB-8csq: EMDB-26968, PDB-8csr: EMDB-26969, PDB-8css: EMDB-26970, PDB-8cst: EMDB-26971, PDB-8csu: EMDB-27249, PDB-8d8j: EMDB-27250, PDB-8d8k: EMDB-27251, PDB-8d8l: |
Chemicals | ChemComp-SF4: ChemComp-SAM: ChemComp-K: ChemComp-MG: ChemComp-FES: ChemComp-ZN: ChemComp-ATP: ChemComp-GDP: ChemComp-NAD: ChemComp-HOH: |
Source |
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Keywords | RIBOSOME / Ribonucleoprotein complex / Mitochondria Biogenesis / Ribonucleoprotein complex Mitochondria Biogenesis |