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TitleA streamlined approach to structure elucidation using in cellulo crystallized recombinant proteins, InCellCryst.
Journal, issue, pagesNat Commun, Vol. 15, Page 1709-1709, Year 2024
Publish dateJan 6, 2023 (structure data deposition date)
AuthorsSchonherr, R. / Boger, J. / Lahey-Rudolph, J.M. / Harms, M. / Kaiser, J. / Nachtschatt, S. / Wobbe, M. / Duden, R. / Konig, P. / Bourenkov, G. ...Schonherr, R. / Boger, J. / Lahey-Rudolph, J.M. / Harms, M. / Kaiser, J. / Nachtschatt, S. / Wobbe, M. / Duden, R. / Konig, P. / Bourenkov, G. / Schneider, T.R. / Redecke, L.
External linksNat Commun / PubMed:38402242
MethodsX-ray diffraction
Resolution1.56 - 3 Å
Structure data

PDB-8c51:
Trypanosoma brucei IMP dehydrogenase (cyto) crystallized in High Five cells revealing native ligands ATP, GDP and phosphate. Diffraction data collection at 100 K in cellulo
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-8c53:
Trypanosoma brucei IMP dehydrogenase (ori) crystallized in High Five cells reveals native ligands ATP, GDP and phosphate. Diffraction data collection at 100 K in cellulo; CrystFEL processing
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-8c5k:
HEX-1 (in cellulo, in situ) crystallized and diffracted in High Five cells. Growth and SX data collection at 296 K on CrystalDirect plates
Method: X-RAY DIFFRACTION / Resolution: 2.16 Å

PDB-8cd4:
structure of HEX-1 from N. crassa crystallized in cellulo (cytosol), diffracted at 100K and resolved using CrystFEL
Method: X-RAY DIFFRACTION / Resolution: 1.83 Å

PDB-8cd5:
structure of HEX-1 from N. crassa crystallized in cellulo, diffracted at 100K and resolved using CrystFEL
Method: X-RAY DIFFRACTION / Resolution: 1.56 Å

PDB-8cd6:
structure of HEX-1 (cyto V2) from N. crassa grown in living insect cells, diffracted at 100K and resolved using CrystFEL
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-8cgx:
structure of HEX-1 from N. crassa crystallized in cellulo, diffracted at 100K and resolved using XDS
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-8cgy:
Trypanosoma brucei IMP dehydrogenase (ori) crystallized in High Five cells reveals native ligands ATP, GDP and phosphate. Diffraction data collection at 100 K in cellulo; XDS processing
Method: X-RAY DIFFRACTION / Resolution: 3 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HOH:
WATER

Source
  • trypanosoma brucei (eukaryote)
  • neurospora crassa or74a (fungus)
  • neurospora crassa (fungus)
KeywordsTRANSFERASE / IMP dehydrogenase / purine biosynthetic enzyme / native cofactors / STRUCTURAL PROTEIN / Woronin body / self-assembly / HEX-1 / in vivo / naturally crystallizing

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