Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	The molybdenum storage protein forms and deposits distinct polynuclear tungsten oxygen aggregates.
Journal, issue, pages	J Inorg Biochem, Vol. 234, Page 111904, Year 2022
Publish date	Jun 16, 2022
Authors	Iram Aziz / Susann Kaltwasser / Kanwal Kayastha / Radhika Khera / Janet Vonck / Ulrich Ermler /
PubMed Abstract	Some N-fixing bacteria store Mo to maintain the formation of the vital FeMo-cofactor dependent nitrogenase under Mo depleting conditions. The Mo storage protein (MoSto), developed for this purpose, ...Some N-fixing bacteria store Mo to maintain the formation of the vital FeMo-cofactor dependent nitrogenase under Mo depleting conditions. The Mo storage protein (MoSto), developed for this purpose, has the unique capability to compactly deposit molybdate as polyoxometalate (POM) clusters in a (αβ) hexameric cage; the same occurs with the physicochemically related tungstate. To explore the structural diversity of W-based POM clusters, MoSto loaded under different conditions with tungstate and two site-specifically modified MoSto variants were structurally characterized by X-ray crystallography or single-particle cryo-EM. The MoSto cage contains five major locations for POM clusters occupied among others by heptanuclear, Keggin ion and even Dawson-like species also found in bulk solvent under defined conditions. We found both lacunary derivatives of these archetypical POM clusters with missing WO units at positions exposed to bulk solvent and expanded derivatives with additional WO units next to protecting polypeptide segments or other POM clusters. The cryo-EM map, unexpectedly, reveals a POM cluster in the cage center anchored to the wall by a WO linker. Interestingly, distinct POM cluster structures can originate from identical, highly occupied core fragments of three to seven WO units that partly correspond to those found in MoSto loaded with molybdate. These core fragments are firmly bound to the complementary protein template in contrast to the more variable, less occupied residual parts of the visible POM clusters. Due to their higher stability, W-based POM clusters are, on average, larger and more diverse than their Mo-based counterparts.
External links	J Inorg Biochem / PubMed:35779405
Methods	EM (single particle) / X-ray diffraction
Resolution	1.4 - 2.58 Å
Structure data	14522 7z5j EMDB-14522, PDB-7z5j: The molybdenum storage protein loaded with tungstate Method: EM (single particle) / Resolution: 2.58 Å PDB-7zqq: Molybdenum storage protein - LB131H Method: X-RAY DIFFRACTION / Resolution: 1.75 Å PDB-7zr4: Molybdenum storage protein loaded with polyoxotungstates in the in vivo-like state Method: X-RAY DIFFRACTION / Resolution: 1.701 Å PDB-7zse: Molybdenum storage protein in complex with polyoxotungstates in the in-vitro state Method: X-RAY DIFFRACTION / Resolution: 1.4 Å
Chemicals	ChemComp-MOO: MOLYBDATE ION ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-IWL: W11-O35 cluster ChemComp-IV9: 1,1,3,3,5,7,7,9,11,15,15-undecakis($l^{1}-oxidanyl)-2$l^{4},4$l^{3},6$l^{5},8,10,12,14,16,17,18,19$l^{3},20,21,22,23-pentadecaoxa-1$l^{6},3$l^{6},5$l^{6},7$l^{6},9$l^{6},11$l^{6},13$l^{6},15$l^{6}-octatungstapentadecacyclo[7.7.1.1^{1,13}.1^{3,5}.1^{3,15}.1^{5,7}.1^{5,11}.1^{7,11}.0^{2,13}.0^{2,15}.0^{4,13}.0^{6,9}.0^{6,11}.0^{6,13}.0^{9,19}]tricosane ChemComp-IWO: W8-O26 cluster ChemComp-IWZ: W10-O37 cluster ChemComp-IHW: tungstate cluster ChemComp-IX3: W3-O10 cluster ChemComp, No image ChemComp-UNL: Unknown ligand ChemComp-CL: Unknown entry ChemComp-PO4: PHOSPHATE ION ChemComp-HOH*: WATER
Source	azotobacter vinelandii dj (bacteria) azotobacter vinelandii (bacteria)
Keywords	METAL BINDING PROTEIN / Metal storage protein / polytungstate / Keggin ion / EM / polyoxotungstate / metal storage / molybdenum storage / polyoxotungstates / Molybdenum storage protein / polyoxotungstate clusters