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TitleMechanism of protein-primed template-independent DNA synthesis by Abi polymerases.
Journal, issue, pagesNucleic Acids Res, Vol. 50, Issue 17, Page 10026-10040, Year 2022
Publish dateSep 23, 2022
AuthorsMałgorzata Figiel / Marta Gapińska / Mariusz Czarnocki-Cieciura / Weronika Zajko / Małgorzata Sroka / Krzysztof Skowronek / Marcin Nowotny
PubMed AbstractAbortive infection (Abi) is a bacterial antiphage defense strategy involving suicide of the infected cell. Some Abi pathways involve polymerases that are related to reverse transcriptases. They are ...Abortive infection (Abi) is a bacterial antiphage defense strategy involving suicide of the infected cell. Some Abi pathways involve polymerases that are related to reverse transcriptases. They are unique in the way they combine the ability to synthesize DNA in a template-independent manner with protein priming. Here, we report crystal and cryo-electron microscopy structures of two Abi polymerases: AbiK and Abi-P2. Both proteins adopt a bilobal structure with an RT-like domain that comprises palm and fingers subdomains and a unique helical domain. AbiK and Abi-P2 adopt a hexameric and trimeric configuration, respectively, which is unprecedented for reverse transcriptases. Biochemical experiments showed that the formation of these oligomers is required for the DNA polymerization activity. The structure of the AbiK-DNA covalent adduct visualized interactions between the 3' end of DNA and the active site and covalent attachment of the 5' end of DNA to a tyrosine residue used for protein priming. Our data reveal a structural basis of the mechanism of highly unusual template-independent protein-priming polymerases.
External linksNucleic Acids Res / PubMed:36107766 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.27 - 3.1 Å
Structure data

14220

7r06

EMDB-14220, PDB-7r06:
Abortive infection DNA polymerase AbiK from Lactococcus lactis
Method: EM (single particle) / Resolution: 2.27 Å

14435

7z0z

EMDB-14435, PDB-7z0z:
Abortive infection DNA polymerase AbiK from Lactococcus lactis, Y44F variant
Method: EM (single particle) / Resolution: 2.68 Å

PDB-7r07:
Abortive infection DNA polymerase AbiK from Lactococcus lactis
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-7r08:
Abortive infection DNA polymerase Abi-P2
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • lactococcus lactis (lactic acid bacteria)
  • escherichia coli (E. coli)
  • escherichia coli bl21 (bacteria)
  • escherichia phage p2 (virus)
KeywordsANTIVIRAL PROTEIN / DNA polymerase / protein-primed DNA synthesis / template-independent DNA synthesis / abortive infection

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