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TitleCrystal Structure of an Archaeal Tyrosyl-tRNA Synthetase Bound to Photocaged L-Tyrosine and Its Potential Application to Time-Resolved X-ray Crystallography.
Journal, issue, pagesInt J Mol Sci, Vol. 23, Year 2022
Publish dateAug 1, 2022 (structure data deposition date)
AuthorsHosaka, T. / Katsura, K. / Ishizuka-Katsura, Y. / Hanada, K. / Ito, K. / Tomabechi, Y. / Inoue, M. / Akasaka, R. / Takemoto, C. / Shirouzu, M.
External linksInt J Mol Sci / PubMed:36142308
MethodsX-ray diffraction
Resolution1.39 - 2.79 Å
Structure data

PDB-7ynu:
Crystal structure of Hen Egg white LYSOZYME introduced with O-(2-nitrobenzyl)-L-tyrosine
Method: X-RAY DIFFRACTION / Resolution: 1.44 Å

PDB-7ynv:
Crystal structure of photolysed Hen Egg white LYSOZYME introduced with O-(2-nitrobenzyl)-L-tyrosine
Method: X-RAY DIFFRACTION / Resolution: 1.39 Å

PDB-7ynw:
Crystal structure of O-(2-nitrobenzyl)-L-tyrosine-tRNA sythetase in complex with O-(2-nitrobenzyl)-L-tyrosine
Method: X-RAY DIFFRACTION / Resolution: 2.79 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-HOH:
WATER

ChemComp-J2F:
(2~{S})-2-azanyl-3-[4-[(2-nitrophenyl)methoxy]phenyl]propanoic acid

Source
  • gallus gallus (chicken)
  • methanocaldococcus jannaschii (archaea)
KeywordsBIOSYNTHETIC PROTEIN / nonnatural amino acid caged amino acid o-(2-nitrobenzyl)-L-tyrosine lysozyme cell-free protein synthesis / TRANSCRIPTION / nonnatural amino acid / cell-free protein synthesis / caged amino acid / o-(2-nitrobenzyl)-L-tyrosine amino-acyl tRNA synthetase

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