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TitleCryo-EM structures of human p97 double hexamer capture potentiated ATPase-competent state.
Journal, issue, pagesCell Discov, Vol. 8, Issue 1, Page 19, Year 2022
Publish dateFeb 22, 2022
AuthorsHaishan Gao / Faxiang Li / Zhejian Ji / Zhubing Shi / Yang Li / Hongtao Yu /
PubMed AbstractThe conserved ATPase p97 (Cdc48 in yeast) and adaptors mediate diverse cellular processes through unfolding polyubiquitinated proteins and extracting them from macromolecular assemblies and membranes ...The conserved ATPase p97 (Cdc48 in yeast) and adaptors mediate diverse cellular processes through unfolding polyubiquitinated proteins and extracting them from macromolecular assemblies and membranes for disaggregation and degradation. The tandem ATPase domains (D1 and D2) of the p97/Cdc48 hexamer form stacked rings. p97/Cdc48 can unfold substrates by threading them through the central pore. The pore loops critical for substrate unfolding are, however, not well-ordered in substrate-free p97/Cdc48 conformations. How p97/Cdc48 organizes its pore loops for substrate engagement is unclear. Here we show that p97/Cdc48 can form double hexamers (DH) connected through the D2 ring. Cryo-EM structures of p97 DH reveal an ATPase-competent conformation with ordered pore loops. The C-terminal extension (CTE) links neighboring D2s in each hexamer and expands the central pore of the D2 ring. Mutations of Cdc48 CTE abolish substrate unfolding. We propose that the p97/Cdc48 DH captures a potentiated state poised for substrate engagement.
External linksCell Discov / PubMed:35190543 / PubMed Central
MethodsEM (single particle)
Resolution3.15 - 3.32 Å
Structure data

31894

7vcs

EMDB-31894, PDB-7vcs:
Human p97 double hexamer conformer II with ATPgammaS bound
Method: EM (single particle) / Resolution: 3.32 Å

31895

7vct

EMDB-31895, PDB-7vct:
Human p97 single hexamer conformer III with D1-ATPgammaS and D2-ADP bound
Method: EM (single particle) / Resolution: 3.21 Å

31896

7vcu

EMDB-31896, PDB-7vcu:
Human p97 double hexamer conformer I with D1-ATPgammaS and D2-ADP bound
Method: EM (single particle) / Resolution: 3.15 Å

31897

7vcv

EMDB-31897, PDB-7vcv:
Human p97 single hexamer conformer I with ATPgammaS bound
Method: EM (single particle) / Resolution: 3.21 Å

31899

7vcx

EMDB-31899, PDB-7vcx:
Human p97 single hexamer conformer II with ATPgammaS bound
Method: EM (single particle) / Resolution: 3.24 Å

Chemicals

ChemComp-AGS:
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

Source
  • homo sapiens (human)
KeywordsHYDROLASE / AAA+ ATPase / unfoldase / CELL CYCLE

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