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TitleCryo-EM structure of an extracellular Geobacter OmcE cytochrome filament reveals tetrahaem packing.
Journal, issue, pagesNat Microbiol, Vol. 7, Issue 8, Page 1291-1300, Year 2022
Publish dateJul 7, 2022
AuthorsFengbin Wang / Khawla Mustafa / Victor Suciu / Komal Joshi / Chi H Chan / Sol Choi / Zhangli Su / Dong Si / Allon I Hochbaum / Edward H Egelman / Daniel R Bond /
PubMed AbstractElectrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens were first observed two decades ago, with genetic and biochemical data suggesting that conductive fibres were ...Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens were first observed two decades ago, with genetic and biochemical data suggesting that conductive fibres were type IV pili. Recently, an extracellular conductive filament of G. sulfurreducens was found to contain polymerized c-type cytochrome OmcS subunits, not pilin subunits. Here we report that G. sulfurreducens also produces a second, thinner appendage comprised of cytochrome OmcE subunits and solve its structure using cryo-electron microscopy at ~4.3 Å resolution. Although OmcE and OmcS subunits have no overall sequence or structural similarities, upon polymerization both form filaments that share a conserved haem packing arrangement in which haems are coordinated by histidines in adjacent subunits. Unlike OmcS filaments, OmcE filaments are highly glycosylated. In extracellular fractions from G. sulfurreducens, we detected type IV pili comprising PilA-N and -C chains, along with abundant B-DNA. OmcE is the second cytochrome filament to be characterized using structural and biophysical methods. We propose that there is a broad class of conductive bacterial appendages with conserved haem packing (rather than sequence homology) that enable long-distance electron transport to chemicals or other microbial cells.
External linksNat Microbiol / PubMed:35798889 / PubMed Central
MethodsEM (helical sym.)
Resolution4.1 - 4.3 Å
Structure data

25879

7tfs

EMDB-25879, PDB-7tfs:
Cryo-EM of the OmcE nanowires from Geobacter sulfurreducens
Method: EM (helical sym.) / Resolution: 4.3 Å

25881

7tgg

EMDB-25881, PDB-7tgg:
Cryo-EM structure of PilA-N and PilA-C from Geobacter sulfurreducens
Method: EM (helical sym.) / Resolution: 4.1 Å

Chemicals

ChemComp-HEC:
HEME C

Source
  • geobacter sulfurreducens (bacteria)
KeywordsELECTRON TRANSPORT / PROTEIN FIBRIL / helical symmetry / cytochrome nanowire / filament / pili / type iv pili / pseudo pili

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