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-Structure paper
タイトル | Cryo-EM structure of an extracellular Geobacter OmcE cytochrome filament reveals tetrahaem packing. |
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ジャーナル・号・ページ | Nat Microbiol, Vol. 7, Issue 8, Page 1291-1300, Year 2022 |
掲載日 | 2022年7月7日 |
著者 | Fengbin Wang / Khawla Mustafa / Victor Suciu / Komal Joshi / Chi H Chan / Sol Choi / Zhangli Su / Dong Si / Allon I Hochbaum / Edward H Egelman / Daniel R Bond / |
PubMed 要旨 | Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens were first observed two decades ago, with genetic and biochemical data suggesting that conductive fibres were ...Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens were first observed two decades ago, with genetic and biochemical data suggesting that conductive fibres were type IV pili. Recently, an extracellular conductive filament of G. sulfurreducens was found to contain polymerized c-type cytochrome OmcS subunits, not pilin subunits. Here we report that G. sulfurreducens also produces a second, thinner appendage comprised of cytochrome OmcE subunits and solve its structure using cryo-electron microscopy at ~4.3 Å resolution. Although OmcE and OmcS subunits have no overall sequence or structural similarities, upon polymerization both form filaments that share a conserved haem packing arrangement in which haems are coordinated by histidines in adjacent subunits. Unlike OmcS filaments, OmcE filaments are highly glycosylated. In extracellular fractions from G. sulfurreducens, we detected type IV pili comprising PilA-N and -C chains, along with abundant B-DNA. OmcE is the second cytochrome filament to be characterized using structural and biophysical methods. We propose that there is a broad class of conductive bacterial appendages with conserved haem packing (rather than sequence homology) that enable long-distance electron transport to chemicals or other microbial cells. |
リンク | Nat Microbiol / PubMed:35798889 / PubMed Central |
手法 | EM (らせん対称) |
解像度 | 4.1 - 4.3 Å |
構造データ | EMDB-25879, PDB-7tfs: EMDB-25881, PDB-7tgg: |
化合物 | ChemComp-HEC: |
由来 |
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キーワード | ELECTRON TRANSPORT / PROTEIN FIBRIL / helical symmetry / cytochrome nanowire / filament / pili / type iv pili / pseudo pili |