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Structure paper

TitleHigh-resolution cryo-electron microscopy structure of photosystem II from the mesophilic cyanobacterium, sp. PCC 6803.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 119, Issue 1, Year 2022
Publish dateJan 4, 2022
AuthorsChristopher J Gisriel / Jimin Wang / Jinchan Liu / David A Flesher / Krystle M Reiss / Hao-Li Huang / Ke R Yang / William H Armstrong / M R Gunner / Victor S Batista / Richard J Debus / Gary W Brudvig /
PubMed AbstractPhotosystem II (PSII) enables global-scale, light-driven water oxidation. Genetic manipulation of PSII from the mesophilic cyanobacterium sp. PCC 6803 has provided insights into the mechanism of ...Photosystem II (PSII) enables global-scale, light-driven water oxidation. Genetic manipulation of PSII from the mesophilic cyanobacterium sp. PCC 6803 has provided insights into the mechanism of water oxidation; however, the lack of a high-resolution structure of oxygen-evolving PSII from this organism has limited the interpretation of biophysical data to models based on structures of thermophilic cyanobacterial PSII. Here, we report the cryo-electron microscopy structure of PSII from sp. PCC 6803 at 1.93-Å resolution. A number of differences are observed relative to thermophilic PSII structures, including the following: the extrinsic subunit PsbQ is maintained, the C terminus of the D1 subunit is flexible, some waters near the active site are partially occupied, and differences in the PsbV subunit block the Large (O1) water channel. These features strongly influence the structural picture of PSII, especially as it pertains to the mechanism of water oxidation.
External linksProc Natl Acad Sci U S A / PubMed:34937700 / PubMed Central
MethodsEM (single particle)
Resolution1.93 - 2.01 Å
Structure data

24239

7n8o

EMDB-24239, PDB-7n8o:
High-resolution structure of photosystem II from the mesophilic cyanobacterium, Synechocystis sp. PCC 6803
Method: EM (single particle) / Resolution: 1.93 Å

24407

7rcv

EMDB-24407, PDB-7rcv:
High-resolution structure of photosystem II from the mesophilic cyanobacterium, Synechocystis sp. PCC 6803
Method: EM (single particle) / Resolution: 2.01 Å

Chemicals

ChemComp-OEX:
CA-MN4-O5 CLUSTER

ChemComp-FE2:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-CLA:
CHLOROPHYLL A

ChemComp-PHO:
PHEOPHYTIN A

ChemComp-BCR:
BETA-CAROTENE

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-PL9:
2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-CA:
Unknown entry

ChemComp-RRX:
(3R)-beta,beta-caroten-3-ol

ChemComp-HOH:
WATER

Source
  • Synechocystis sp. PCC 6803 (bacteria)
  • synechocystis sp. (strain pcc 6803 / kazusa) (bacteria)
KeywordsPHOTOSYNTHESIS / Photosystem II / Cyanobacteria / Synechocystis 6803 / Mesophile / CyanoQ

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