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Title | Structural and biochemical characterization of human Schlafen 5. |
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Journal, issue, pages | Nucleic Acids Res, Vol. 50, Issue 2, Page 1147-1161, Year 2022 |
Publish date | Jan 25, 2022 |
Authors | Felix J Metzner / Elisabeth Huber / Karl-Peter Hopfner / Katja Lammens / |
PubMed Abstract | The Schlafen family belongs to the interferon-stimulated genes and its members are involved in cell cycle regulation, T cell quiescence, inhibition of viral replication, DNA-repair and tRNA ...The Schlafen family belongs to the interferon-stimulated genes and its members are involved in cell cycle regulation, T cell quiescence, inhibition of viral replication, DNA-repair and tRNA processing. Here, we present the cryo-EM structure of full-length human Schlafen 5 (SLFN5) and the high-resolution crystal structure of the highly conserved N-terminal core domain. We show that the core domain does not resemble an ATPase-like fold and neither binds nor hydrolyzes ATP. SLFN5 binds tRNA as well as single- and double-stranded DNA, suggesting a potential role in transcriptional regulation. Unlike rat Slfn13 or human SLFN11, human SLFN5 did not cleave tRNA. Based on the structure, we identified two residues in proximity to the zinc finger motif that decreased DNA binding when mutated. These results indicate that Schlafen proteins have divergent enzymatic functions and provide a structural platform for future biochemical and genetic studies. |
External links | Nucleic Acids Res / PubMed:35037067 / PubMed Central |
Methods | EM (single particle) / X-ray diffraction |
Resolution | 1.85 - 3.44 Å |
Structure data | EMDB-13581, PDB-7ppj: PDB-6rr9: PDB-7q3z: |
Chemicals | ChemComp-ZN: ChemComp-SO4: ChemComp-GOL: ChemComp-NA: ChemComp-HOH: |
Source |
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Keywords | DNA BINDING PROTEIN / Zinc-finger protein / RNA-binding / nucleotide binding protein antiviral linked to tumorigenesis transcription regulation |