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Structure paper

TitleStructure of photosystem II reveals conformational flexibility of stacked and unstacked supercomplexes.
Journal, issue, pagesElife, Vol. 12, Year 2023
Publish dateFeb 17, 2023
AuthorsIdo Caspy / Maria Fadeeva / Yuval Mazor / Nathan Nelson /
PubMed AbstractPhotosystem II (PSII) generates an oxidant whose redox potential is high enough to enable water oxidation , a substrate so abundant that it assures a practically unlimited electron source for life on ...Photosystem II (PSII) generates an oxidant whose redox potential is high enough to enable water oxidation , a substrate so abundant that it assures a practically unlimited electron source for life on earth . Our knowledge on the mechanism of water photooxidation was greatly advanced by high-resolution structures of prokaryotic PSII . Here, we show high-resolution cryogenic electron microscopy (cryo-EM) structures of eukaryotic PSII from the green alga at two distinct conformations. The conformers are also present in stacked PSII, exhibiting flexibility that may be relevant to the grana formation in chloroplasts of the green lineage. CP29, one of PSII associated light-harvesting antennae, plays a major role in distinguishing the two conformations of the supercomplex. We also show that the stacked PSII dimer, a form suggested to support the organisation of thylakoid membranes , can appear in many different orientations providing a flexible stacking mechanism for the arrangement of grana stacks in thylakoids. Our findings provide a structural basis for the heterogenous nature of the eukaryotic PSII on multiple levels.
External linksElife / PubMed:36799903 / PubMed Central
MethodsEM (single particle)
Resolution2.43 - 4.0 Å
Structure data

13429

7pi0

EMDB-13429, PDB-7pi0:
Unstacked compact Dunaliella PSII
Method: EM (single particle) / Resolution: 2.43 Å

13430

7pi5

EMDB-13430, PDB-7pi5:
Unstacked stretched Dunaliella PSII
Method: EM (single particle) / Resolution: 2.78 Å

13444

7pin

EMDB-13444, PDB-7pin:
Stacked compact Dunaliella PSII
Method: EM (single particle) / Resolution: 3.6 Å

13455

7piw

EMDB-13455, PDB-7piw:
Stacked stretched Dunaliella PSII
Method: EM (single particle) / Resolution: 4.0 Å

13548

7pnk

EMDB-13548, PDB-7pnk:
Unstacked compact Dunaliella PSII
Method: EM (single particle) / Resolution: 3.61 Å

Chemicals

ChemComp-OEX:
CA-MN4-O5 CLUSTER

ChemComp-FE2:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-CLA:
CHLOROPHYLL A

ChemComp-PHO:
PHEOPHYTIN A

ChemComp-BCR:
BETA-CAROTENE

ChemComp-NA:
Unknown entry

ChemComp-3PH:
1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE

ChemComp-C7Z:
(1~{S})-3,5,5-trimethyl-4-[(1~{E},3~{E},5~{E},7~{E},9~{E},11~{E},13~{E},15~{E},17~{E})-3,7,12,16-tetramethyl-18-[(4~{S})-2,6,6-trimethyl-4-oxidanyl-cyclohexen-1-yl]octadeca-1,3,5,7,9,11,13,15,17-nonaenyl]cyclohex-3-en-1-ol

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

ChemComp-SPH:
SPHINGOSINE

ChemComp-DGA:
DIACYL GLYCEROL

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM

ChemComp-PL9:
2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-RRX:
(3R)-beta,beta-caroten-3-ol

ChemComp-CHL:
CHLOROPHYLL B

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

ChemComp-PTY:
PHOSPHATIDYLETHANOLAMINE / phospholipid*YM

ChemComp-LPX:
(2S)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-hydroxypropyl hexadecanoate

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

ChemComp-LMK:
trimethyl-[(2~{R})-1-oxidanyl-1-oxidanylidene-4-[(2~{S})-2-[(1~{S})-1-oxidanyloctadecoxy]-3-[(1~{R})-1-oxidanyloctadecoxy]propoxy]butan-2-yl]azanium

ChemComp-4RF:
Tripalmitoylglycerol

ChemComp-ERG:
ERGOSTEROL

Source
  • dunaliella salina (plant)
KeywordsPHOTOSYNTHESIS / green algae / photosystem II / thylakoid / oxygen evolving complex / cryo-EM / stacking

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