+Open data
-Basic information
Entry | Database: PDB / ID: 7pi0 | |||||||||
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Title | Unstacked compact Dunaliella PSII | |||||||||
Components |
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Keywords | PHOTOSYNTHESIS / green algae / photosystem II / thylakoid / oxygen evolving complex / cryo-EM / stacking | |||||||||
Function / homology | Function and homology information photosynthesis, light harvesting / photosystem II stabilization / oxygen evolving activity / photosystem II / photosystem II reaction center / photosynthetic electron transport chain / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosystem I / response to herbicide / photosystem II ...photosynthesis, light harvesting / photosystem II stabilization / oxygen evolving activity / photosystem II / photosystem II reaction center / photosynthetic electron transport chain / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosystem I / response to herbicide / photosystem II / photosynthetic electron transport in photosystem II / chlorophyll binding / chloroplast thylakoid membrane / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / phosphate ion binding / photosynthesis / electron transfer activity / protein stabilization / iron ion binding / heme binding / metal ion binding Similarity search - Function | |||||||||
Biological species | Dunaliella salina (plant) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.43 Å | |||||||||
Authors | Caspy, I. / Fadeeva, M. / Mazor, Y. / Nelson, N. | |||||||||
Funding support | Israel, 2items
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Citation | Journal: Elife / Year: 2023 Title: Structure of photosystem II reveals conformational flexibility of stacked and unstacked supercomplexes. Authors: Ido Caspy / Maria Fadeeva / Yuval Mazor / Nathan Nelson / Abstract: Photosystem II (PSII) generates an oxidant whose redox potential is high enough to enable water oxidation , a substrate so abundant that it assures a practically unlimited electron source for life on ...Photosystem II (PSII) generates an oxidant whose redox potential is high enough to enable water oxidation , a substrate so abundant that it assures a practically unlimited electron source for life on earth . Our knowledge on the mechanism of water photooxidation was greatly advanced by high-resolution structures of prokaryotic PSII . Here, we show high-resolution cryogenic electron microscopy (cryo-EM) structures of eukaryotic PSII from the green alga at two distinct conformations. The conformers are also present in stacked PSII, exhibiting flexibility that may be relevant to the grana formation in chloroplasts of the green lineage. CP29, one of PSII associated light-harvesting antennae, plays a major role in distinguishing the two conformations of the supercomplex. We also show that the stacked PSII dimer, a form suggested to support the organisation of thylakoid membranes , can appear in many different orientations providing a flexible stacking mechanism for the arrangement of grana stacks in thylakoids. Our findings provide a structural basis for the heterogenous nature of the eukaryotic PSII on multiple levels. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7pi0.cif.gz | 1.6 MB | Display | PDBx/mmCIF format |
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PDB format | pdb7pi0.ent.gz | 1.5 MB | Display | PDB format |
PDBx/mmJSON format | 7pi0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pi/7pi0 ftp://data.pdbj.org/pub/pdb/validation_reports/pi/7pi0 | HTTPS FTP |
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-Related structure data
Related structure data | 13429MC 7pi5C 7pinC 7piwC 7pnkC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
-Photosystem II ... , 12 types, 24 molecules AaBbVvCcDdHhIiJjKkLlTtZz
#1: Protein | Mass: 37291.488 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: D0FY08, photosystem II #2: Protein | Mass: 53321.516 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: A0A1C8XRM6 #3: Protein/peptide | Mass: 3217.949 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: D0FY13 #4: Protein | Mass: 49128.992 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: D0FXY3 #5: Protein | Mass: 38965.383 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: A0A1C8XRK9, photosystem II #8: Protein | Mass: 7124.381 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: A0A1C8XRP3 #9: Protein/peptide | Mass: 3929.648 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: D0FXX5 #10: Protein/peptide | Mass: 3737.500 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: A0A1C8XRM8 #11: Protein/peptide | Mass: 4159.991 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: D0FXX2 #12: Protein/peptide | Mass: 4417.203 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: D0FY19 #16: Protein/peptide | Mass: 3478.194 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: D0FY04 #19: Protein | Mass: 6430.616 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: D0FXZ2 |
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-Cytochrome b559 subunit ... , 2 types, 4 molecules EeFf
#6: Protein | Mass: 8743.847 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: D0FY01 #7: Protein/peptide | Mass: 3549.299 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: A0A1C8XRP4 |
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-Protein/peptide , 4 types, 8 molecules MmWwXxUu
#13: Protein/peptide | Mass: 3307.979 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) #17: Protein/peptide | Mass: 4698.315 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) #18: Protein/peptide | Mass: 2854.407 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) #25: Protein/peptide | Mass: 3219.625 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) |
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-Protein , 7 types, 14 molecules OoPpNnGgRrSsYy
#14: Protein | Mass: 25874.908 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) #15: Protein | Mass: 20425.707 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) #20: Protein | Mass: 24075.082 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) #21: Protein | Mass: 23719.543 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) / References: UniProt: A1XKU7 #22: Protein | Mass: 21163.662 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) #23: Protein | Mass: 26233.607 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) #24: Protein | Mass: 23537.566 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Dunaliella salina (plant) |
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-Sugars , 2 types, 10 molecules
#37: Sugar | ChemComp-DGD / #49: Sugar | |
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