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TitleStructures and implications of TBP-nucleosome complexes.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 118, Issue 30, Year 2021
Publish dateJul 27, 2021
AuthorsHaibo Wang / Le Xiong / Patrick Cramer /
PubMed AbstractThe TATA box-binding protein (TBP) is highly conserved throughout eukaryotes and plays a central role in the assembly of the transcription preinitiation complex (PIC) at gene promoters. TBP binds and ...The TATA box-binding protein (TBP) is highly conserved throughout eukaryotes and plays a central role in the assembly of the transcription preinitiation complex (PIC) at gene promoters. TBP binds and bends DNA, and directs adjacent binding of the transcription factors TFIIA and TFIIB for PIC assembly. Here, we show that yeast TBP can bind to a nucleosome containing the Widom-601 sequence and that TBP-nucleosome binding is stabilized by TFIIA. We determine three cryo-electron microscopy (cryo-EM) structures of TBP-nucleosome complexes, two of them containing also TFIIA. TBP can bind to superhelical location (SHL) -6, which contains a TATA-like sequence, but also to SHL +2, which is GC-rich. Whereas binding to SHL -6 can occur in the absence of TFIIA, binding to SHL +2 is only observed in the presence of TFIIA and goes along with detachment of upstream terminal DNA from the histone octamer. TBP-nucleosome complexes are sterically incompatible with PIC assembly, explaining why a promoter nucleosome generally impairs transcription and must be moved before initiation can occur.
External linksProc Natl Acad Sci U S A / PubMed:34301908 / PubMed Central
MethodsEM (single particle)
Resolution2.5 - 3.4 Å
Structure data

12897

7oh9

EMDB-12897, PDB-7oh9:
Nucleosome with TBP and TFIIA bound at SHL -6
Method: EM (single particle) / Resolution: 3.0 Å

12898

7oha

EMDB-12898, PDB-7oha:
nucleosome with TBP and TFIIA bound at SHL +2
Method: EM (single particle) / Resolution: 2.9 Å

12899

7ohb

EMDB-12899, PDB-7ohb:
TBP-nucleosome complex
Method: EM (single particle) / Resolution: 3.4 Å

12900

7ohc

EMDB-12900, PDB-7ohc:
Cryo-EM structure of nucleosome core particle composed of the Widom 601 DNA sequence
Method: EM (single particle) / Resolution: 2.5 Å

Source
  • xenopus laevis (African clawed frog)
  • synthetic construct (others)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSCRIPTION / DNA binding protein nucleosome / NUCLEAR PROTEIN / nucleosome

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