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Structure paper

TitleStructural insights into photosystem II assembly.
Journal, issue, pagesNat Plants, Vol. 7, Issue 4, Page 524-538, Year 2021
Publish dateApr 12, 2021
AuthorsJure Zabret / Stefan Bohn / Sandra K Schuller / Oliver Arnolds / Madeline Möller / Jakob Meier-Credo / Pasqual Liauw / Aaron Chan / Emad Tajkhorshid / Julian D Langer / Raphael Stoll / Anja Krieger-Liszkay / Benjamin D Engel / Till Rudack / Jan M Schuller / Marc M Nowaczyk /
PubMed AbstractBiogenesis of photosystem II (PSII), nature's water-splitting catalyst, is assisted by auxiliary proteins that form transient complexes with PSII components to facilitate stepwise assembly events. ...Biogenesis of photosystem II (PSII), nature's water-splitting catalyst, is assisted by auxiliary proteins that form transient complexes with PSII components to facilitate stepwise assembly events. Using cryo-electron microscopy, we solved the structure of such a PSII assembly intermediate from Thermosynechococcus elongatus at 2.94 Å resolution. It contains three assembly factors (Psb27, Psb28 and Psb34) and provides detailed insights into their molecular function. Binding of Psb28 induces large conformational changes at the PSII acceptor side, which distort the binding pocket of the mobile quinone (Q) and replace the bicarbonate ligand of non-haem iron with glutamate, a structural motif found in reaction centres of non-oxygenic photosynthetic bacteria. These results reveal mechanisms that protect PSII from damage during biogenesis until water splitting is activated. Our structure further demonstrates how the PSII active site is prepared for the incorporation of the MnCaO cluster, which performs the unique water-splitting reaction.
External linksNat Plants / PubMed:33846594 / PubMed Central
MethodsEM (single particle)
Resolution2.66 - 2.72 Å
Structure data

12335

7nho

EMDB-12335, PDB-7nho:
Structure of PSII-M
Method: EM (single particle) / Resolution: 2.66 Å

12336

7nhp

EMDB-12336, PDB-7nhp:
Structure of PSII-I (PSII with Psb27, Psb28, and Psb34)
Method: EM (single particle) / Resolution: 2.72 Å

12337

7nhq

EMDB-12337, PDB-7nhq:
Structure of PSII-I prime (PSII with Psb28, and Psb34)
Method: EM (single particle) / Resolution: 2.68 Å

Chemicals

ChemComp-FE:
Unknown entry / Iron

ChemComp-MN:
Unknown entry

ChemComp-CL:
Unknown entry / Chloride

ChemComp-PHO:
PHEOPHYTIN A / Pheophytin

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM / Bicarbonate

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-PL9:
2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE / Plastoquinone

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

Source
  • thermosynechococcus elongatus bp-1 (bacteria)
KeywordsPHOTOSYNTHESIS / Membrane Protein Biogenesis Assembly Factors Photosystem II

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