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TitleDeterministic chaos in the self-assembly of β sheet nanotubes from an amphipathic oligopeptide.
Journal, issue, pagesMatter, Vol. 4, Issue 10, Page 3217-3231, Year 2021
Publish dateOct 6, 2021
AuthorsFengbin Wang / Ordy Gnewou / Shengyuan Wang / Tomasz Osinski / Xiaobing Zuo / Edward H Egelman / Vincent P Conticello /
PubMed AbstractThe self-assembly of designed peptides into filaments and other higher-order structures has been the focus of intense interest because of the potential for creating new biomaterials and biomedical ...The self-assembly of designed peptides into filaments and other higher-order structures has been the focus of intense interest because of the potential for creating new biomaterials and biomedical devices. These peptide assemblies have also been used as models for understanding biological processes, such as the pathological formation of amyloid. We investigate the assembly of an octapeptide sequence, Ac-FKFEFKFE-NH, motivated by prior studies that demonstrated that this amphipathic β strand peptide self-assembled into fibrils and biocompatible hydrogels. Using high-resolution cryoelectron microscopy (cryo-EM), we are able to determine the atomic structure for two different coexisting forms of the fibrils, containing four and five β sandwich protofilaments, respectively. Surprisingly, the inner walls in both forms are parallel β sheets, while the outer walls are antiparallel β sheets. Our results demonstrate the chaotic nature of peptide self-assembly and illustrate the importance of cryo-EM structural analysis to understand the complex phase behavior of these materials at near-atomic resolution.
External linksMatter / PubMed:34632372 / PubMed Central
MethodsEM (helical sym.)
Resolution3.4 - 3.8 Å
Structure data

23483

7lqe

EMDB-23483, PDB-7lqe:
Cryo-EM of 1-protofilament of the KFE8 thinner nanotube
Method: EM (helical sym.) / Resolution: 3.4 Å

23484

7lqf

EMDB-23484, PDB-7lqf:
Cryo-EM of KFE8 ribbon filament
Method: EM (helical sym.) / Resolution: 3.8 Å

23485

7lqg

EMDB-23485, PDB-7lqg:
Cryo-EM of the KFE8 thinner nanotube (class 1, C2)
Method: EM (helical sym.) / Resolution: 3.4 Å

23486

7lqh

EMDB-23486, PDB-7lqh:
Cryo-EM of KFE8 thinner nanotube (class 2, 2-sub-1)
Method: EM (helical sym.) / Resolution: 3.4 Å

23487

7lqi

EMDB-23487, PDB-7lqi:
Cryo-EM of KFE8 thicker nanotube
Method: EM (helical sym.) / Resolution: 3.5 Å

Source
  • synthetic construct (others)
KeywordsPROTEIN FIBRIL / filament / self-assembly peptide filament / Cryo-EM / peptide fibril / nanotube

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