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- PDB-7lqf: Cryo-EM of KFE8 ribbon filament -

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Basic information

Entry
Database: PDB / ID: 7lqf
TitleCryo-EM of KFE8 ribbon filament
ComponentsKFE8 peptide
KeywordsPROTEIN FIBRIL / filament / self-assembly peptide filament / Cryo-EM / peptide fibril / nanotube
Biological speciessynthetic construct (others)
MethodELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsWang, F. / Gnewou, O.M. / Egelman, E.H. / Conticello, V.P.
Funding support United States, 1items
OrganizationGrant numberCountry
National Science Foundation (NSF, United States)NSF-DMR-1533958 United States
CitationJournal: Matter / Year: 2021
Title: Deterministic chaos in the self-assembly of β sheet nanotubes from an amphipathic oligopeptide.
Authors: Fengbin Wang / Ordy Gnewou / Shengyuan Wang / Tomasz Osinski / Xiaobing Zuo / Edward H Egelman / Vincent P Conticello /
Abstract: The self-assembly of designed peptides into filaments and other higher-order structures has been the focus of intense interest because of the potential for creating new biomaterials and biomedical ...The self-assembly of designed peptides into filaments and other higher-order structures has been the focus of intense interest because of the potential for creating new biomaterials and biomedical devices. These peptide assemblies have also been used as models for understanding biological processes, such as the pathological formation of amyloid. We investigate the assembly of an octapeptide sequence, Ac-FKFEFKFE-NH, motivated by prior studies that demonstrated that this amphipathic β strand peptide self-assembled into fibrils and biocompatible hydrogels. Using high-resolution cryoelectron microscopy (cryo-EM), we are able to determine the atomic structure for two different coexisting forms of the fibrils, containing four and five β sandwich protofilaments, respectively. Surprisingly, the inner walls in both forms are parallel β sheets, while the outer walls are antiparallel β sheets. Our results demonstrate the chaotic nature of peptide self-assembly and illustrate the importance of cryo-EM structural analysis to understand the complex phase behavior of these materials at near-atomic resolution.
History
DepositionFeb 13, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 30, 2021Provider: repository / Type: Initial release
Revision 1.1Aug 11, 2021Group: Database references / Category: citation / citation_author / database_2
Item: _citation.journal_abbrev / _citation.journal_id_CSD ..._citation.journal_abbrev / _citation.journal_id_CSD / _citation.pdbx_database_id_DOI / _citation.title / _citation.year / _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.2Oct 27, 2021Group: Database references / Category: citation / citation_author
Item: _citation.journal_id_ISSN / _citation.journal_volume ..._citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID
Revision 2.0Nov 15, 2023Group: Atomic model / Data collection / Category: atom_site / chem_comp_atom / chem_comp_bond / Item: _atom_site.auth_atom_id / _atom_site.label_atom_id

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Structure visualization

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Assembly

Deposited unit
A: KFE8 peptide
I: KFE8 peptide
J: KFE8 peptide
K: KFE8 peptide
B: KFE8 peptide
L: KFE8 peptide
M: KFE8 peptide
N: KFE8 peptide
C: KFE8 peptide
O: KFE8 peptide
P: KFE8 peptide
Q: KFE8 peptide
D: KFE8 peptide
R: KFE8 peptide
S: KFE8 peptide
T: KFE8 peptide
E: KFE8 peptide
U: KFE8 peptide
V: KFE8 peptide
W: KFE8 peptide
F: KFE8 peptide
X: KFE8 peptide
Y: KFE8 peptide
Z: KFE8 peptide
G: KFE8 peptide
i: KFE8 peptide
j: KFE8 peptide
k: KFE8 peptide
H: KFE8 peptide
l: KFE8 peptide
m: KFE8 peptide
n: KFE8 peptide
a: KFE8 peptide
o: KFE8 peptide
p: KFE8 peptide
q: KFE8 peptide
b: KFE8 peptide
r: KFE8 peptide
s: KFE8 peptide
t: KFE8 peptide
c: KFE8 peptide
u: KFE8 peptide
v: KFE8 peptide
w: KFE8 peptide
d: KFE8 peptide
x: KFE8 peptide
y: KFE8 peptide
z: KFE8 peptide
e: KFE8 peptide
0: KFE8 peptide
1: KFE8 peptide
2: KFE8 peptide
f: KFE8 peptide
3: KFE8 peptide
4: KFE8 peptide
5: KFE8 peptide
g: KFE8 peptide
6: KFE8 peptide
7: KFE8 peptide
8: KFE8 peptide
h: KFE8 peptide
9: KFE8 peptide
AA: KFE8 peptide
BA: KFE8 peptide


Theoretical massNumber of molelcules
Total (without water)74,51864
Polymers74,51864
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: microscopy, helical filament was observed by negative staining and Cryo-EM
TypeNameSymmetry operationNumber
identity operation1_5551
SymmetryHelical symmetry: (Circular symmetry: 1 / Dyad axis: no / N subunits divisor: 1 / Num. of operations: 64 / Rise per n subunits: 7.93 Å / Rotation per n subunits: -15.8 °)

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Components

#1: Protein/peptide ...
KFE8 peptide


Mass: 1164.350 Da / Num. of mol.: 64 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: helical reconstruction

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Sample preparation

ComponentName: self-assembly KFE8 nanotubes / Type: COMPLEX / Entity ID: all / Source: NATURAL
Source (natural)Organism: synthetic construct (others)
Buffer solutionpH: 4
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

SoftwareName: PHENIX / Version: 1.16_3549: / Classification: refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Helical symmertyAngular rotation/subunit: -15.8 ° / Axial rise/subunit: 7.93 Å / Axial symmetry: C1
3D reconstructionResolution: 3.8 Å / Resolution method: OTHER / Num. of particles: 26198 / Details: Model:Map FSC 0.38 cut off / Symmetry type: HELICAL
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0076264
ELECTRON MICROSCOPYf_angle_d0.9118136
ELECTRON MICROSCOPYf_dihedral_angle_d19.6083096
ELECTRON MICROSCOPYf_chiral_restr0.054576
ELECTRON MICROSCOPYf_plane_restr0.0021080

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